Genotoxicity of gemfibrozil in the gilthead seabream (Sparus aurata)

被引：16

作者：

Barreto, A. ^{[1
,2
]}

Luis, L. G. ^{[1
,2
]}

Soares, A. M. V. M. ^{[1
,2
]}

Paiga, P. ^{[3
]}

Santos, L. H. M. L. M. ^{[3
]}

Delerue-Matos, C. ^{[3
]}

Hylland, K. ^{[4
]}

Loureiro, S. ^{[1
,2
]}

Oliveira, M. ^{[1
,2
]}

机构：

[1] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal

[3] Inst Politecn Porto, Inst Super Engn Porto, Requimte LAQV, Rua Dr Antonio Bernardino de Almeida 431, P-4200072 Oporto, Portugal

[4] Univ Oslo, Dept Biosci, POB 1066, N-0316 Oslo, Norway

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2017年 / 821卷

关键词：

Gemfibrozil; Seabream; DNA damage; Comet assay; Erythrocyte abnormalities; Micronucleus assay; ERYTHROCYTIC NUCLEAR ABNORMALITIES; COMET ASSAY MEASUREMENTS; OXIDATIVE DNA-DAMAGE; SHORT-TERM EXPOSURE; MICRONUCLEUS TEST; LIPID REGULATOR; LIZA-AURATA; PHARMACEUTICALS GEMFIBROZIL; PERIPHERAL ERYTHROCYTES; GREY MULLET;

D O I：

10.1016/j.mrgentox.2017.05.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Widespread use of pharmaceuticals and suboptimal wastewater treatment have led to increased levels of these substances in aquatic ecosystems. Lipid-lowering drugs such as gemfibrozil, which are among the most abundant human pharmaceuticals in the environment, may have deleterious effects on aquatic organisms. We examined the genotoxicity of gemfibrozil in a fish species, the gilthead seabream (Sparta aurata), which is commercially important in southern Europe. Following 96-h waterborne exposure, molecular (erythrocyte DNA strand breaks) and cytogenetic (micronuclei and other nuclear abnormalities in cells) endpoints were measured. Gemfibrozil was positive in both endpoints, at environmentally relevant concentrations, a result that raises concerns about the potential genotoxic effects of the drug in recipient waters.

引用

页码：36 / 42

页数：7

共 81 条

[1] Cytotoxic and genotoxic responses of the RTgill-W1 fish cells in combination with the yeast oestrogen screen to determine the sediment quality of Lagos lagoon, Nigeria [J].

Amaeze, Nnamdi H. ;

Schnell, Sabine ;

Sozeri, Osman ;

Otitoloju, Adebayo A. ;

Egonmwan, Rosemary I. ;

Arlt, Volker M. ;

Bury, Nic R. .

MUTAGENESIS, 2015, 30 (01) :117-127

[2] Pharmaceuticals in STP effluents and their solar photodegradation in aquatic environment [J].

Andreozzi, R ;

Marotta, R ;

Paxeus, N .

CHEMOSPHERE, 2003, 50 (10) :1319-1330

[3] Induction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna:: an assessment of the fish micronucleus test [J].

Ayllon, F ;

Garcia-Vazquez, EG .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2000, 467 (02) :177-186

[4] Comet assay to measure DNA repair: approach and applications [J].

Azqueta, Amaya ;

Slyskova, Jana ;

Langie, Sabine A. S. ;

Gaivao, Isabel O'Neill ;

Collins, Andrew .

FRONTIERS IN GENETICS, 2014, 5

[5] DNA oxidation: Investigating its key role in environmental mutagenesis with the comet assay [J].

Azqueta, Amaya ;

Shaposhnikov, Sergey ;

Collins, Andrew R. .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2009, 674 (1-2) :101-108

[6]

Bargiene J., 2014, ENVIRON MONIT ASSESS, V187, P1

[7] Assessment of micronuclei induction in peripheral erythrocytes of fish exposed to xenobiotics under controlled conditions [J].

Bolognesi, Claudia ;

Perrone, Emanuela ;

Roggieri, Paola ;

Pampanin, Daniela M. ;

Sciutto, Andrea .

AQUATIC TOXICOLOGY, 2006, 78 :S93-S98

[8] Application of the comet and micronucleus assays to butterfish (Pholis gunnellus) erythrocytes from the Firth of Forth, Scotland [J].

Bombail, V ;

Aw, D ;

Gordon, E ;

Batty, J .

CHEMOSPHERE, 2001, 44 (03) :383-392

[9] Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization [J].

Buschini, A ;

Martino, A ;

Gustavino, B ;

Monfrinotti, M ;

Poli, P ;

Rossi, C ;

Santoro, A ;

Dörr, AM ;

Rizzoni, M .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2004, 557 (02) :119-129

[10] Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries-Portugal [J].

Carrola, Joao ;

Santos, Nadia ;

Rocha, Maria J. ;

Fontainhas-Fernandes, Antonio ;

Pardal, Miguel A. ;

Monteiro, Rogerio A. F. ;

Rocha, Eduardo .

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2014, 21 (09) :6057-6068

← 1 2 3 4 5 6 7 8 9 →