Genome-wide identification and expression analysis of aquaporin gene family related to abiotic stress in watermelon

被引:23
作者
Zhou, Yong [1 ]
Tao, Junjie [1 ,2 ]
Ahammed, Golam Jalal [3 ]
Li, Jingwen [1 ,2 ]
Yang, Youxin [1 ,2 ]
机构
[1] Jiangxi Agr Univ, Minist Educ, Key Lab Crop Physiol Ecol & Genet Breeding, Nanchang 330045, Jiangxi, Peoples R China
[2] Jiangxi Agr Univ, Coll Agron, Dept Hort, Nanchang 330045, Jiangxi, Peoples R China
[3] Henan Univ Sci & Technol, Coll Forestry, Luoyang 471023, Peoples R China
基金
中国国家自然科学基金;
关键词
watermelon; aquaporin (AQP); gene structure; expression profile; abiotic stress; MAJOR INTRINSIC PROTEINS; PLASMA-MEMBRANE AQUAPORIN; ARABIDOPSIS; TOLERANCE; PLANTS; OVEREXPRESSION; RESPONSES; DROUGHT; SILICON; BORON;
D O I
10.1139/gen-2019-0061
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The plant aquaporins (AQPs) are highly conserved integral membrane proteins that participate in multiple developmental processes and responses to various stresses. In this study, a total of 35 AQP genes were identified in the watermelon genome. The phylogenetic analysis showed that these AQPs can be divided into five types, including 16 plasma membrane intrinsic proteins (PIPs), eight tonoplast intrinsic proteins (TIPs), eight nodulin 26-like intrinsic proteins (NIPs), two small basic intrinsic proteins (SIPs), and one uncategorized X intrinsic protein (XIP). A number of cis-elements related to plant responses to hormones and stresses were detected in the promoter sequences of ClAQP genes. Chromosome distribution analysis revealed that the genes are unevenly distributed on eight chromosomes, with chromosomes 1 and 4 possessing the most genes. Expression analysis at different developmental stages in flesh and rind indicated that most of ClAQPs have tissue-specific expression. Meanwhile, some other AQP genes showed differential expression in response to cold, salt, and ABA treatments, which is consistent with the organization of the stress-responsive cis-elements detected in the promoter regions. Our results lay a foundation for understanding the specific functions of ClAQP genes to help the genetic improvement of watermelon.
引用
收藏
页码:643 / 656
页数:14
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