The pH dependence of Escherichia coli O157:H7 adsorption on kaolinite and goethite surfaces

被引：11

作者：

Zhang, Taoxiang ^{[1
]}

Yang, Wenhao ^{[1
]}

Zhu, Xinyu ^{[1
]}

Wang, Haizhen ^{[1
]}

Brookes, Philip C. ^{[1
]}

Xu, Jianming ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Hangzhou 310058, Zhejiang, Peoples R China

来源：

JOURNAL OF SOILS AND SEDIMENTS | 2015年 / 15卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Adsorption; Kaolinite and goethite; E. coli O157:H7; pH; ENTERICA SEROVAR TYPHIMURIUM; ATR-FTIR SPECTROSCOPY; PACKED-BED COLUMN; SALMONELLA-ENTERICA; BACILLUS-SUBTILIS; CLAY-MINERALS; PAENIBACILLUS-POLYMYXA; SOLUTION CHEMISTRY; BACTERIAL-CELLS; IONIC-STRENGTH;

D O I：

10.1007/s11368-014-0948-7

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Understanding the adsorption processes of Escherichia coli O157:H7 in the soil-water system is important in order to protect public health from waterborne diseases. The aim of this study was to investigate the role of pH on E. coli O157:H7 adsorption on kaolinite and goethite. The adsorption of E. coli O157:H7 on kaolinite and goethite over a wide range of pH levels (3-10) was determined. Confocal microscopy, zeta potential, classic Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, and Fourier transform infrared spectrometry were used to provide insights into the mechanisms that affect E. coli O157:H7 adsorption. E. coli O157:H7 adsorption was greater on the positively charged goethite than on the negatively charged kaolinite. The maximum adsorption of E. coli O157:H7 occurred on goethite at pH 3, and increasing pH resulted in decreased adsorption on kaolinite and goethite. Confocal microscopy images confirmed that higher affinities of E. coli O157:H7 occurred on kaolinite and goethite at low pH. Zeta potential, interaction energy, and Fourier transform infrared analysis indicated that electrostatic forces between the E. coli O157:H7 and goethite surfaces controlled the extent of E. coli O157:H7 adsorbed onto goethite surfaces at low pH, and hydrogen bonding and inner-sphere complexation play majors roles in E. coli O157:H7 adsorption to goethite at high pH levels. However, electrostatic force is not the main force for E. coli O157:H7 adsorption to kaolinite. Goethite displayed a larger adsorption capacity for E. coli O157:H7 than kaolinite. The adsorption of bacteria on kaolinite and goethite was pH dependent, and high percentages of E. coli O157:H7 adsorption to kaolinite and goethite occurred at low pH levels.

引用

页码：106 / 116

页数：11

共 49 条

[1] Experimental measurements of the adsorption of Bacillus subtilis and Pseudomonas mendocina onto Fe-oxyhydroxide-coated and uncoated quartz grains [J].