SARS-CoV-2 specific T cell and humoral immune responses upon vaccination with BNT162b2: a 9 months longitudinal study

被引:18
作者
Takeuchi, Junko S. [1 ]
Fukunaga, Ami [2 ]
Yamamoto, Shohei [2 ]
Tanaka, Akihito [3 ]
Matsuda, Kouki [4 ]
Kimura, Moto [1 ]
Kamikawa, Azusa [1 ]
Kito, Yumiko [1 ]
Maeda, Kenji [5 ,6 ]
Ueda, Gohzoh [7 ]
Mizoue, Tetsuya [2 ]
Ujiie, Mugen [8 ]
Mitsuya, Hiroaki [5 ]
Ohmagari, Norio [8 ]
Sugiura, Wataru [9 ]
机构
[1] Natl Ctr Global Hlth & Med, Ctr Clin Sci, Dept Acad Ind Partnerships Promot, Tokyo 1628655, Japan
[2] Natl Ctr Global Hlth & Med, Ctr Clin Sci, Dept Epidemiol & Prevent, Tokyo 1628655, Japan
[3] Ctr Hosp Natl Ctr Global Hlth & Med, Dept Lab Testing, Tokyo 1628655, Japan
[4] Natl Ctr Global Hlth & Med, AIDS Clin Ctr, Tokyo 1628655, Japan
[5] Natl Ctr Global Hlth & Med, Res Inst, Dept Refractory Viral Infect, Tokyo 1628655, Japan
[6] Kagoshima Univ, Joint Res Ctr Human Retrovirus Infect, Div Antiviral Therapy, Kagoshima 8908544, Japan
[7] Abbott Japan LLC, Div Core Diagnost, Tokyo 1086305, Japan
[8] Natl Ctr Global Hlth & Med, Dis Control & Prevent Ctr, Tokyo 1628655, Japan
[9] Natl Ctr Global Hlth & Med, Ctr Clin Sci, Shinjuku Ku, 1-21-1 Toyama, Tokyo 1628655, Japan
关键词
D O I
10.1038/s41598-022-19581-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The humoral and cellular immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upon the coronavirus disease 2019 (COVID-19) vaccination remain to be clarified. Hence, we aimed to investigate the long-term chronological changes in SARS-CoV-2 specific IgG antibody, neutralizing antibody, and T cell responses during and after receiving the BNT162b2 vaccine. We performed serological, neutralization, and T cell assays among 100 hospital workers aged 22-73 years who received the vaccine. We conducted seven surveys up to 8 months after the second vaccination dose. SARS-CoV-2 spike protein-specific IgG (IgG-S) titers and T cell responses increased significantly following the first vaccination dose. The highest titers were observed on day 29 and decreased gradually until the end of the follow-up period. There was no correlation between IgG-S and T cell responses. Notably, T cell responses were detected on day 15, earlier than the onset of neutralizing activity. This study demonstrated that both IgG-S and T cell responses were detected before acquiring sufficient levels of SARS-CoV-2 neutralizing antibodies. These immune responses are sustained for approximately 6 to 10 weeks but not for 7 months or later following the second vaccination, indicating the need for the booster dose (i.e., third vaccination).
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页数:9
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