Synthesis, characterization of α-amino acid Schiff base derived Ru/Pt complexes: Induces cytotoxicity in HepG2 cell via protein binding and ROS generation

被引：35

作者：

Alsalme, Ali ^{[1
]}

Laeeq, Sameen ^{[2
]}

Dwivedi, Sourabh ^{[3
]}

Khan, Mohd. Shahnawaz ^{[4
]}

Al Farhan, Khalid ^{[2
]}

Musarrat, Javed ^{[3
]}

Khan, Rais Ahmad ^{[1
]}

机构：

[1] King Saud Univ, Dept Chem, Coll Sci, POB 2455, Riyadh 11451, Saudi Arabia

[2] Aligarh Muslim Univ, Fac Engn & Technol, Dept Appl Chem, Aligarh 202002, Uttar Pradesh, India

[3] Aligarh Muslim Univ, Fac Agr Sci, Dept Agr Microbiol, Aligarh 202002, Uttar Pradesh, India

[4] King Saud Univ, Dept Biochem, Coll Sci, Riyadh 11451, Saudi Arabia

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2016年 / 163卷

关键词：

Metal complexes; HSA binding; MTT assay; ROS generation; HUMAN SERUM-ALBUMIN; IN-VITRO; PLATINUM(II) COMPLEXES; DIHYDRORHODAMINE; 123; CRYSTAL-STRUCTURE; METAL-COMPLEXES; DNA INTERACTION; DRUG; H2O2;

D O I：

10.1016/j.saa.2016.03.012

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

We have synthesized two new complexes of platinum (1) and ruthenium (2) with alpha-amino acid, L-alanine, and 2,3-dihydroxybenzaldehyde derived Schiff base (L). The ligand and both complexes were characterized by using elemental analysis and several other spectroscopic techniques viz; IR, H-1, C-13 NMR, EPR, and ESI-MS. Furthermore, the protein-binding ability of synthesized complexes was monitored by UV-visible, fluorescence and circular dichroism techniques with a model protein, human serum albumin (HSA). Both the PtL2 and RuL2 complexes displayed significant binding towards HSA. Also, in vitro cytotoxicity assay for both complexes was carried out on human hepatocellular carcinoma cancer (HepG2) cell line. The results showed concentration dependent inhibition of cell viability. Moreover, the generation of reactive oxygen species was also evaluated, and results exhibited substantial role in cytotoxicity. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：1 / 7

页数：7

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