Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

被引：15

作者：

Culebras, Mario ^{[1
,2
]}

Grande, Cristian J. ^{[3
]}

Torres, Fernando G. ^{[3
]}

Troncoso, Omar P. ^{[3
]}

Gomez, Clara M. ^{[1
,2
]}

Carmen Bano, M. ^{[2
,4
]}

机构：

[1] Univ Valencia, Dept Quim Fis, E-46100 Burjassot, Spain

[2] Univ Valencia, Inst Ciencia Mat, E-46100 Burjassot, Spain

[3] Pontificia Univ Catolica Peru, Dept Mech Engn, Lima 32, Peru

[4] Univ Valencia, Fac Biol, Dept Bioquim Biol Mol 1, E-46100 Burjassot, Spain

来源：

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS | 2015年 / 64卷 / 08期

关键词：

Bacterial cellulose; cell growth; poly-L-lysine; collagen; tissue engeneering; MICROBIAL CELLULOSE; MORPHOLOGICAL CHARACTERIZATION; POTENTIAL SCAFFOLD; IN-VIVO; ADHESION; ACID; BIOCOMPATIBILITY; COMPOSITE; SUBSTRATE; MEMBRANES;

D O I：

10.1080/00914037.2014.958829

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates. [GRAPHICS] .

引用

页码：411 / 415

页数：5

共 52 条

[41] Investigation of cancer cell behavior on nanofibrous scaffolds
Szot, Christopher S.
Buchanan, Cara F.
Gatenholm, Paul
Rylander, Marissa Nichole
Freeman, Joseph W.
[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2011, 31 (01): : 37 - 42
[42] Comparison of Biomechanical Properties of Native Menisci and Bacterial Cellulose Implant
Tanaka, Martin L.
Vest, Nikolaj
Ferguson, Cristin M.
Gatenholm, Paul
[J]. INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, 2014, 63 (17) : 891 - 897
[43] Morphological characterisation of natural fibre reinforced thermoplastics (NFRTP) processed by extrusion, compression and rotational moulding
Torres, FG
Díaz, RM
[J]. POLYMERS & POLYMER COMPOSITES, 2004, 12 (08) : 705 - 718
[44] Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties
Ul-Islam, Mazhar
Khan, Taous
Khattak, Waleed Ahmad
Park, Joong Kon
[J]. CELLULOSE, 2013, 20 (02) : 589 - 596
[45] Surface Engineered Polymeric Biomaterials with Improved Biocontact Properties
Vladkova, Todorka G.
[J]. INTERNATIONAL JOURNAL OF POLYMER SCIENCE, 2010, 2010
[46] A NEW BACTERIAL CELLULOSE SUBSTRATE FOR MAMMALIAN-CELL CULTURE - A NEW BACTERIAL CELLULOSE SUBSTRATE
WATANABE, K
ETO, Y
TAKANO, S
NAKAMORI, S
SHIBAI, H
YAMANAKA, S
[J]. CYTOTECHNOLOGY, 1993, 13 (02) : 107 - 114
[47] Preparation and evaluation of a kind of bacterial cellulose dry films with antibacterial properties
Wei, Bin
Yang, Guang
Hong, Feng
[J]. CARBOHYDRATE POLYMERS, 2011, 84 (01) : 533 - 538
[48] Preliminary Results of Small Arterial Substitute Performed with a New Cylindrical Biomaterial Composed of Bacterial Cellulose
Wippermann, J.
Schumann, D.
Klemm, D.
Kosmehl, H.
Satehi-Gelani, S.
Wahlers, T.
[J]. EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY, 2009, 37 (05) : 592 - 596
[49] Antimicrobial activity of silver nanoparticle impregnated bacterial cellulose membrane: Effect of fermentation carbon sources of bacterial cellulose
Yang, Guang
Xie, Jianjian
Hong, Feng
Cao, Zhangjun
Yang, Xuexia
[J]. CARBOHYDRATE POLYMERS, 2012, 87 (01) : 839 - 845
[50] Microporous bacterial cellulose as a potential scaffold for bone regeneration
Zaborowska, Magdalena
Bodin, Aase
Backdahl, Henrik
Popp, Jenni
Goldstein, Aaron
Gatenholm, Paul
[J]. ACTA BIOMATERIALIA, 2010, 6 (07) : 2540 - 2547

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