Expression level of PLAC1 in osteosarcoma patients and its regulatory effect on the development of osteosarcoma

被引:0
作者
Yu, Yifan [1 ]
Xu, Yong [1 ]
Cai, Wei [1 ]
Zuo, Wenshan [1 ]
机构
[1] Nanjing Med Univ, Affiliated Huaian Peoples Hosp 1, Dept Orthopaed, 1 Huanghe West Rd, Huaian 223300, Jiangsu, Peoples R China
来源
JOURNAL OF BUON | 2021年 / 26卷 / 02期
关键词
osteosarcoma; PLAC1; biomarker; mechanism; CELLS; PROLIFERATION; INDUCTION; MIGRATION; INVASION; TARGET;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: To detect the plasma levels of PLAC1 in osteosarcoma patients, and its regulatory effect on cell proliferation and apoptosis of osteosarcoma. Methods: Plasma levels of PLAC1 in osteosarcoma patients were examined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Receiver operating characteristics (ROC) and Kaplan-Meier curves were performed for assessing the diagnostic and prognostic potentials of PLAC1 in osteosarcoma, respectively. Moreover, the regulatory effects of PLAC1 on proliferative and apoptotic rates of osteosarcoma cells were determined through cell counting kit-8 (CCK-8) and flow cytometry, respectively. Results: PLAC1 was highly expressed in plasma of osteosarcoma patients showing diagnostic and prognostic potentials. Overexpression of PLAC1 in U2-OS cells increased the proliferative rate but decreased the apoptotic rate, while knockdown of PLAC1 yielded the opposite results. Conclusions: PLAC1 is upregulated in the plasma of osteosarcoma patients, serving as a diagnostic biomarker, and is unfavorable to the prognosis if this disease. PLAC1 promotes the development of osteosarcoma by stimulating cell proliferation and inhibiting apoptosis.
引用
收藏
页码:592 / 598
页数:7
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