Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis

被引：15

作者：

Liu, Ya-Fei ^{[1
,2
]}

Wen, Cai-Yu-Zhu ^{[3
]}

Chen, Zhe ^{[1
]}

Wang, Yu ^{[1
]}

Huang, Ying ^{[1
]}

Hu, Yong-Hong ^{[1
]}

Tu, Sheng-Hao ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Inst Integrated Tradit Chinese & Western Med, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China

[2] Zhengzhou Univ, Dept Nephrol, Affiliated Hosp 1, 1 Jianshe East Rd, Zhengzhou 450052, Henan, Peoples R China

[3] Hubei Univ Chinese Med, 1 Huangjiahu West Rd, Wuhan 430065, Hubei, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2016年 / 2016卷

基金：

中国国家自然科学基金;

关键词：

RHEUMATOID-ARTHRITIS; EPIGENETIC MODIFICATIONS; DEACETYLASES; PRINCIPLES; ACTIVATION; EXPRESSION; MEDICINE; MBD2;

D O I：

10.1155/2016/5836879

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Background. Wutou decoction (WTD) has been wildly applied in the treatment of rheumatoid arthritis and experimental arthritis in rats for many years. Epigenetic deregulation is associated with the aetiology of rheumatoid arthritis; however, the effects of WTD on epigenetic changes are unclear. This study is set to explore the effects of WTD on DNA methylation and histone modifications in rats with collagen-induced arthritis (CIA). Methods. The CIA model was established by the stimulation of collagen and adjuvant. The knee synovium was stained with hematoxylin and eosin. The DNA methyltransferase 1 (DNMT1) and methylated CpG binding domain 2 (MBD2) expression of peripheral blood mononuclear cells (PBMCs) were determined by Real-Time PCR. The global DNA histone H3-K4/H3-K27 methylation and total histones H3 and H4 acetylation of PBMCs were detected. Results. Our data demonstrated that the DNMT1 mRNA expression was significantly lowered in group WTD compared to that in group CIA (P < 0.05). The DNA methylation level was significantly reduced in group WTD compared to that in group CIA (P < 0.05). Moreover, H3 acetylation of PBMCs was overexpressed in WTD compared with CIA (P < 0.05). Conclusions. WTD may modulate DNA methylation and histone modifications, functioning as anti-inflammatory potential.

引用

页数：9

共 39 条

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