Hyperglycemia and Some Aspects of Leukocyte Activation In Vitro

被引：6

作者：

Mikhalchik, E., V ^{[1
]}

Lipatova, V. A. ^{[2
]}

Basyreva, L. Yu ^{[1
]}

Panasenko, O. M. ^{[1
]}

Gusev, S. A. ^{[1
]}

Sergienko, V., I ^{[1
]}

机构：

[1] Fed Med Biol Agcy Russia, Fed Res & Clin Ctr Phys Chem Med, Moscow, Russia

[2] NI Pirogov Russian Natl Res Med Univ, Minist Hlth Russian Federat, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2021年 / 170卷 / 06期

基金：

俄罗斯科学基金会;

关键词：

diabetes mellitus; neutrophils; priming; chemiluminescence; neutrophil extracellular traps; NEUTROPHILS;

D O I：

10.1007/s10517-021-05147-x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We analyzed functional status of blood leukocytes in diabetes mellitus and after addition of glucose in vitro. To this end, generation of ROS and reactive halogen species by monocytes and neutrophils from patients with diabetes mellitus and healthy donors was assayed using lucigenin- and luminol-dependent chemiluminescence after stimulation with phorbol 12-myristate 13-acetate or opsonized zymosan in vitro. Formation of neutrophil extracellular traps was evaluated in the blood after addition of glucose. In comparison with donors, leukocytes from patients with diabetes mellitus were primed and this effect can be modeled by addition of glucose to the blood in vitro. Addition of glucose to donor blood also triggered the formation of neutrophil extracellular traps.

引用

页码：748 / 751

页数：4

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