Peripheral blood gene expression of B7 and CD28 family members associated with tumor progression and microscopic lymphovascular invasion in colon cancer patients

被引:41
作者
Lee, Hyunji [1 ]
Kim, Ji Hyun [2 ]
Yang, Sung Yeun [2 ]
Kong, Jihye [1 ]
Oh, Minkyung [3 ]
Jeong, Dae Hoon [4 ,6 ]
Chung, Jae-il [5 ]
Bae, Ki Beom [6 ]
Shin, Jin Yong [6 ]
Hong, Kwan Hee [6 ]
Choi, Inhak [1 ]
机构
[1] Inje Univ Coll Med, Dept Microbiol & Immunol, Biomarker Res Ctr Personalized Therapy, Pusan 614735, South Korea
[2] Inje Univ Coll Med, Div Gastroenterol, Dept Internal Med, Pusan 614735, South Korea
[3] Inje Univ Coll Med, Clin Trial Ctr, Busan Paik Hosp, Pusan 614735, South Korea
[4] Inje Univ Coll Med, Dept Obstet & Gynecol, Pusan 614735, South Korea
[5] Inje Univ Coll Med, Dept Urol, Busan Paik Hosp, Pusan 614735, South Korea
[6] Inje Univ Coll Med, Dept Surg, Busan Paik Hosp, Pusan 614735, South Korea
关键词
B7/CD28; family; Peripheral blood transcript; Tumor progression; Lymphovascular invasion; RENAL-CELL CARCINOMA; CLINICAL-SIGNIFICANCE; PROSTATE-CANCER; T-CELLS; B7-H1; MOLECULE; PROGNOSIS; INFECTION; BLOCKADE; IMMUNITY;
D O I
10.1007/s00432-010-0800-4
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To associate the global gene expression of B7/CD28 family transcripts with pathologic features of colon cancer, we determined the B7/CD28 family transcripts in peripheral blood mononuclear cells (PBMCs) from normal subjects and patients with adenomatous polyps and colon cancer, and correlated the results with pathologic features of colon cancer. PBMCs from age-matched normal subjects and patients with adenomatous polyps and colon cancer were analyzed for peripheral blood transcripts (PBTs) of B7/CD28 family using real-time PCR. Differences in expression levels of B7/CD28 PBTs across all cancer stages and between colon cancer patients with or without microscopic lymphovascular invasion (LVI) were analyzed. The results showed a significant upregulation of PBTs of co-inhibitory molecules such as B7-H3 and PD-1 and a significant PBT downregulation of co-stimulatory molecules including CD28 and ICOS in colon cancer patients. Furthermore, the increase of B7-H3 PBT was strongly associated with tumor invasion (P = 0.025) and advanced TNM stages (P = 0.019), whereas the decline of co-stimulatory ligand B7-H2 PBT was related to regional lymph node metastasis (P = 0.028) and aggressive tumor invasion (P = 0.031). In addition, the ratios of PBT expression of CD28 family to B7 family such as CTLA-4 to B7-H2 and PD-1 to B7-H2 were significantly higher in colon cancer patients with microscopic LVI than in those without LVI (P = 0.001 and P = 0.016, respectively). Our results suggest that B7/CD28 family PBTs may serve as valuable markers reflecting the pathological features of colon cancer.
引用
收藏
页码:1445 / 1452
页数:8
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