Impact of oocyte pre-incubation time on fertilization, embryo quality and pregnancy rate after intracytoplasmic sperm injection

被引:44
作者
Isiklar, A [1 ]
Mercan, R [1 ]
Balaban, B [1 ]
Alatas, C [1 ]
Aksoy, S [1 ]
Urman, B [1 ]
机构
[1] Amer Hosp Istanbul, Dept Reprod Med, Istanbul, Turkey
关键词
embryo quality; fertilization rate; pregnancy rate; pre-incubation time;
D O I
10.1016/S1472-6483(10)61649-5
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Although, it is well known that pre-incubation of oocytes prior to conventional IVF improves fertilization and pregnancy rates, there are conflicting results regarding the effect of pre-incubation time in ICSI. This study evaluated the role of preincubation of oocytes on outcome in intracytoplasmic sperm injection (ICSI) cycles. A total of 1260 patients undergoing their first ICSI cycle's were evaluated retrospectively. In patients undergoing ICSI during the year 2000 (Group I, n = 670), oocytes were injected immediately after retrieval, whereas in patients undergoing ICSI during 2001 (Group II, n = 590), oocytes were incubated for 2-4 h prior to injection. The mean age of patients was 33.9 +/- 5.04 years and 34.1 +/- 5.06 years in groups I and II, respectively. The number of oocytes with a first polar body (MII) and fertilization and cleavage rates were higher, and embryo quality was significantly better in group II. In contrast, the total numbers of oocytes without a first polar body (MI), those where germinal vesicle breakdown had not occurred (GV), and empty zona oocytes were higher in group I. No difference was found in the number of embryos transferred or implantation or clinical pregnancy rates. This study demonstrated that pre-incubation of oocytes prior to ICSI is associated with improved maturation of oocytes, fertilization and embryo quality.
引用
收藏
页码:682 / 686
页数:5
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