SILVA, RDP, Greengenes, NCBI and OTT - how do these taxonomies compare?

被引:287
作者
Balvociute, Monika [1 ]
Huson, Daniel H. [1 ]
机构
[1] Univ Tubingen, Dept Comp Sci, Sand 14, D-72076 Tubingen, Germany
关键词
Metagenomics; Taxonomic classification; OTU assignment; NCBI; Silva; RDP; Greengenes; Open tree of life; CLASSIFICATION; EUKARYOTES;
D O I
10.1186/s12864-017-3501-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: A key step in microbiome sequencing analysis is read assignment to taxonomic units. This is often performed using one of four taxonomic classifications, namely SILVA, RDP, Greengenes or NCBI. It is unclear how similar these are and how to compare analysis results that are based on different taxonomies. Results: We provide a method and software for mapping taxonomic entities from one taxonomy onto another. We use it to compare the four taxonomies and the Open Tree of life Taxonomy (OTT). Conclusions: While we find that SILVA, RDP and Greengenes map well into NCBI, and all four map well into the OTT, mapping the two larger taxonomies on to the smaller ones is problematic.
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页数:8
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