Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires

被引:37
作者
Kohsaka, T
Hamano, K
Sasada, H
Watanabe, S
Ogine, T
Suzuki, E
Nishida, S
Takahara, H
Sato, E
机构
[1] Shizuoka Univ, Lab Anim Reprod, Fac Agr, Shizuoka 4228529, Japan
[2] Shinshu Univ, Fac Agr, Res Farm, Nagano 3994598, Japan
[3] Tohoku Univ, Lab Anim Reprod, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan
[4] Minist Agr Forestry & Fisheries, Agr Forestry & Fisheries Res Council, Tokyo 1008950, Japan
[5] Cent Res Inst Feed & Livestock Zen Noh, Ibaraki 3000000, Japan
[6] Miyagi Livestock Expt Stn, Sendai, Miyagi 98964, Japan
[7] Ibaraki Univ, Sch Agr, Lab Mol Biol Chem, Ibaraki 3000332, Japan
来源
INTERNATIONAL JOURNAL OF ANDROLOGY | 2003年 / 26卷 / 02期
关键词
domestic animals; motility; relaxin; seminal plasma; sperm;
D O I
10.1046/j.1365-2605.2003.00409.x
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Although immunoassayable relaxin has been detected in human and boar seminal plasma, there is no evidence suggesting the existence of immunoreactive relaxin in the seminal plasma of other domestic animals. The first objective of this study was to determine whether immunoreactive relaxin was present in the seminal plasma of bulls, rams and he-goats. In addition, the correlation of immunoreactive relaxin with sperm motility as an index for predicting the fertilizing ability of bull sires was investigated. Semen with normal sperm motility was collected from bulls, rams and he-goats, and the relaxin immunoreactivity of the semen samples was measured using a time-resolved fluoroimmunoassay (TR-FIA) for porcine relaxin that we developed. The presence of relaxin immunoreactivity was demonstrated in seminal plasma from bulls, rams and he-goats. The level of immunoreactive relaxin in seminal plasma was the highest in bulls followed by humans, rams, boars and he-goats in that order, when relaxin levels in boar and human semen having normal sperm motility were also assayed under the same conditions. When the correlation between the seminal plasma level of immunoreactive relaxin and sperm motility was examined in bull semen samples as an index for predicting fertilizing ability, it was found that the relaxin level was significantly correlated with the percentage of spermatozoa showing the most intensive motility (r = 0.64, p < 0.05). These results indicate that immunoreactive relaxin is widely found in the seminal plasma of domestic animals and that measuring the relaxin concentration of seminal plasma may be useful to identify subfertile sires or predict the fertility potential of individual sires.
引用
收藏
页码:115 / 120
页数:6
相关论文
共 33 条
[1]  
ACOTT TS, 1978, J BIOL CHEM, V253, P6744
[2]   SPERM FORWARD MOTILITY PROTEIN - TISSUE DISTRIBUTION AND SPECIES CROSS-REACTIVITY [J].
ACOTT, TS ;
JOHNSON, DJ ;
BRANDT, H ;
HOSKINS, DD .
BIOLOGY OF REPRODUCTION, 1979, 20 (02) :247-252
[3]  
BRENNER SH, 1987, FERTIL STERIL, V47, P714
[4]   THE BINDING OF RECOMBINANT HUMAN RELAXIN TO HUMAN SPERMATOZOA [J].
CARRELL, DT ;
PETERSON, CM ;
URRY, RL .
ENDOCRINE RESEARCH, 1995, 21 (03) :697-707
[5]  
COLON JM, 1986, FERTIL STERIL, V46, P1133
[6]  
DECOOMAN S, 1983, FERTIL STERIL, V39, P111
[7]  
ESSIG M, 1982, FERTIL STERIL, V38, P339
[8]   EXPRESSION OF THE HUMAN RELAXIN H1-GENE IN THE DECIDUA, TROPHOBLAST, AND PROSTATE [J].
HANSELL, DJ ;
BRYANTGREENWOOD, GD ;
GREENWOOD, FC .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1991, 72 (04) :899-904
[9]   EXPRESSION OF THE HUMAN RELAXIN GENE IN THE CORPUS-LUTEUM OF THE MENSTRUAL-CYCLE AND IN THE PROSTATE [J].
IVELL, R ;
HUNT, N ;
KHANDAWOOD, F ;
DAWOOD, MY .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1989, 66 (02) :251-255
[10]   EFFECT OF RELAXIN AND ANTIRELAXIN SERUM ON PORCINE SPERM MOTILITY [J].
JUANG, HH ;
MUSAH, AI ;
SCHWABE, C ;
ANDERSON, LL .
ANIMAL REPRODUCTION SCIENCE, 1989, 20 (01) :21-29