The Production of Transgenic Carrot Plants Resistant to Herbicide Basta

被引：0

作者：

Sidorova, T. ^{[1
]}

Miroshnichenko, D. ^{[1
]}

Dolgov, S. ^{[1
]}

Tjukavin, G. ^{[2
]}

机构：

[1] RAS, Branch Inst Bioorgan Chem, Biotron, Pushchino, Moscow Region, Russia

[2] RAAS, All Russian Res Inst Vegetable Breeding & Seed Pr, Lesnoy Gorodok, Moscow Region, Russia

来源：

VIII INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURAL BREEDING | 2015年 / 1083卷

关键词：

Agrobacterium; bar gene; herbicide resistance; phosphinothricin acetyl transferase;

D O I：

10.17660/ActaHortic.2015.1083.14

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A construct containing two chimeric genes, Cauliflower mosaic virus 35s - (bar) and nopaline synthase -neomycin phosphotransferase (nptII), was introduced into carrot cultivar 'Nantskaya 4' using Agrobacterium tumefaciens-mediated transformation procedure. A number of transgenic carrot plants showing the stable integration of the bar gene were produced from embryogenic callus culture. Most of the obtained transgenic plants showed complete herbicide resistance at 0.05-0.50% Basta. Thus, introduction of the bar gene into a carrot cultivar can allow efficient production of herbicide-resistant transgenic carrot with commercial potential.

引用

页码：133 / 137

页数：5

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