The role of N-methyl-D-aspartate receptor subunit NR2B in spinal cord in cancer pain

被引：59

作者：

Gu, XiaoPing ^{[1
]}

Zhang, Juan ^{[1
]}

Ma, ZhengLiang ^{[1
]}

Wang, JunHua ^{[1
]}

Zhou, XiaoFang ^{[1
]}

Jin, YanQing ^{[1
]}

Xia, XiaoPing ^{[1
]}

Gao, Qin ^{[1
]}

Mei, FengMei ^{[1
]}

机构：

[1] Nanjing Univ, Affiliated Drum Tower Hosp, Dept Anesthesiol, Coll Med, Nanjing 210008, Peoples R China

来源：

EUROPEAN JOURNAL OF PAIN | 2010年 / 14卷 / 05期

关键词：

NR2B; Cancer pain; Ifenprodil; Mouse model; NMDA receptor; DORSAL-HORN NEURONS; NMDA RECEPTORS; NEUROPATHIC PAIN; CENTRAL SENSITIZATION; FUNCTIONAL-PROPERTIES; SENSORY NEURONS; MURINE MODEL; NERVE INJURY; BONE PAIN; RAT;

D O I：

10.1016/j.ejpain.2009.09.001

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

Cancer pain is one kind of the most common and severe kinds of chronic pain. No breakthrough regarding the mechanisms and therapeutics of cancer pains has yet been achieved. Based on the well established involvement of the NMDA (N-methyl-D-aspartate) receptor containing NR2B in inflammatory pain and neuropathic pain and the effective pain relief obtained with ketamine in cancer patients with intractable pain, we supposed that NR2B in the spinal cord was an important factor for cancer pain. In this study, we investigated the possible role of NR2B in the spinal cord using a murine model of bone cancer pain. C3H/HeJ mice were inoculated into the intramedullary space of the right femur with Osteosarcoma NCTC 2472 cells to induce ongoing bone cancer-related pain behaviors. At day 14 after operation, the expression of NR2B mRNA and NR2B protein in the spinal cord were higher in tumor-bearing mice compared to the sham mice. Intrathecal administration of 5 and 10 mu g of NR2B subunit-specific NMDA receptor antagonist ifenprodil attenuated cancer-evoked spontaneous pain, thermal hyperalgesia and mechanical allodynia. These results suggest that NR2B in the spinal cord may participate in bone cancer pain in mice, and ifenprodil may be a useful alternative or adjunct therapy for bone cancer pain. The findings may lead to novel strategies for the treatment of bone cancer pain. (C) 2009 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved.

引用

页码：496 / 502

页数：7