Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep

被引:26
作者
Balseiro, Ana [1 ]
Altuzarra, Raul [2 ]
Vidal, Enric [3 ]
Moll, Xavier [2 ]
Espada, Yvonne [2 ]
Sevilla, Iker A. [4 ]
Domingo, Mariano [3 ,5 ]
Garrido, Joseba M. [4 ]
Juste, Ramon A. [1 ]
Prieto, Miguel [1 ]
Perez de Val, Bernat [3 ]
机构
[1] SERIDA, Serv Reg Invest & Desarrollo Agroalimentario, Ctr Biotecnol Anim, Gijon, Asturias, Spain
[2] Univ Autonoma Barcelona, Dept Med & Cirugia Anim, Barcelona, Catalonia, Spain
[3] UAB, Ctr Recerca Sanitat Anim CReSA, IRTA, Campus Univ Autanoma Barcelona, Barcelona, Spain
[4] NEIKER Tecnalia, Inst Vasco Invest & Desarrollo Agr, Dept Sanidad Anim, Bizkaia, Pais Vasco, Spain
[5] Univ Autonoma Barcelona, Dept Sanitat & Anat Anim, Barcelona, Catalonia, Spain
来源
PLOS ONE | 2017年 / 12卷 / 07期
关键词
CALMETTE-GUERIN VACCINATION; DEER ODOCOILEUS-VIRGINIANUS; ORAL VACCINATION; ANTIBODY-RESPONSES; BRUSHTAIL POSSUMS; CATTLE; BACILLUS; BADGERS; PROTECTION; PATHOLOGY;
D O I
10.1371/journal.pone.0180546
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background/Aims Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 x 10(3) CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate.
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页数:16
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