Cloning and characterization of a cDNA fragment coding β-casein-like protein preferentially expressed in cervical adenocarcinoma cell line CAC-1

被引：4

作者：

Suzuki, T ^{[1
]}

Koizumi, M ^{[1
]}

Baba, T ^{[1
]}

Kudo, R ^{[1
]}

机构：

[1] Sapporo Med Univ, Dept Obstet & Gynecol, Chuo Ku, Sapporo, Hokkaido 060, Japan

来源：

CANCER LETTERS | 1998年 / 124卷 / 02期

关键词：

cDNA fragment; beta-casein; CAC-1;

D O I：

10.1016/S0304-3835(97)00471-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We generated new murine monoclonal antibodies against bovine beta-casein. One of eight new monoclonal antibodies generated, 12G2, specifically reacted not only with beta-casein, but also with human cervical adenocarcinoma cell line CAC-1. To identify the gene encoding the putative protein (BCLP) defined by monoclonal antibody (mAb) 12G2, a complementary DNA library was constructed. We obtained the inserted cDNA fragment that showed high homology to reported DNA sequences of unknown function in normal human placenta. We think that BCLP was overexpressed or changed its structure by carcinogenesis. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：165 / 171

页数：7

共 20 条

[1] BETA-CASEIN MESSENGER-RNA SEQUESTERS A SINGLE-STRANDED NUCLEIC ACID-BINDING PROTEIN WHICH NEGATIVELY REGULATES THE BETA-CASEIN GENE PROMOTER
ALTIOK, S
GRONER, B
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (09) : 6004 - 6012
[2] COW MILK-PROTEINS - 25 YEARS OF PROGRESS
BRUNNER, JR
[J]. JOURNAL OF DAIRY SCIENCE, 1981, 64 (06) : 1038 - 1054
[3] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[4] COHEN C, 1993, ARCH PATHOL LAB MED, V117, P291
[5] RAT-X-RAT HYBRID MYELOMAS AND A MONOCLONAL ANTI-FD PORTION OF MOUSE IGG
GALFRE, G
MILSTEIN, C
WRIGHT, B
[J]. NATURE, 1979, 277 (5692) : 131 - 133
[6] HAYAKAWA O, 1988, Sapporo Medical Journal, V57, P603
[7] Horimoto E, 1994, Asia Oceania J Obstet Gynaecol, V20, P321
[8] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY
KOHLER, G
MILSTEIN, C
[J]. NATURE, 1975, 256 (5517) : 495 - 497
[9] KOHRI K, 1993, J BIOL CHEM, V268, P15180
[10] KOIZUMI M, 1988, CANCER RES, V48, P6565

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