Augmented lipid accumulation in ethyl methyl sulphonate mutants of oleaginous microalga for biodiesel production

被引：29

作者：

Mehtani, Juhi ^{[1
]}

Arora, Neha ^{[1
]}

Patel, Alok ^{[1
]}

Jain, Priyanka ^{[2
]}

Pruthi, Parul A. ^{[1
]}

Poluri, Kirshna Mohan ^{[1
]}

Pruthi, Vikas ^{[1
]}

机构：

[1] Indian Inst Technol Roorkee, Dept Biotechnol, Roorkee, Uttarakhand, India

[2] Indian Inst Technol Roorkee, Dept Chem Engn, Roorkee 247667, Uttarakhand, India

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 242卷

关键词：

Chlorella minutissima; Ethyl Methyl Sulphonate; Lipid content; Mutant; Triacylglycerol; STARCH-DEFICIENT MUTANTS; CHLAMYDOMONAS-REINHARDTII; CHLORELLA-PYRENOIDOSA; RANDOM MUTAGENESIS; BIOMASS; ENHANCEMENT; CAROTENOIDS; MINUTISSIMA; MUTATION; GENES;

D O I：

10.1016/j.biortech.2017.03.108

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The aim of this work was to generate high lipid accumulating mutants of Chlorella minutissima (CM) using ethyl methyl sulphonate (EMS) as a random chemical mutagen. Amid the 5% surviving cells after exposure to EMS (2 M), three fast growing mutants (CM2, CM5, CM7) were selected and compared with wild type for lipid productivity and biochemical composition. Among these mutants, CM7 showed the maximum biomass (2.4 g/L) and lipid content (42%) as compared to wild type (1.5 g/L; 27%). Further, the mutant showed high photosynthetic pigments with low starch content signifying the re-allocation of carbon flux to lipid. The obtained mutant showed no visible morphological changes in comparison to its WT. The fatty acid profile showed increase in monounsaturated fatty acids while decreased saturated and polyunsaturated fatty acids signifying good quality biodiesel. The mutant strain thus obtained can be optimized further and applied for enhanced biodiesel production. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：121 / 127

页数：7

共 34 条

[1] Characterization of microalga Nannochloropsis sp mutants for improved production of biofuels [J].

Anandarajah, Kandiah ;

Mahendraperumal, Guruvaiah ;

Sommerfeld, Milton ;

Hu, Qiang .

APPLIED ENERGY, 2012, 96 :371-377

[2] Boosting TAG Accumulation with Improved Biodiesel Production from Novel Oleaginous Microalgae Scenedesmus sp IITRIND2 Utilizing Waste Sugarcane Bagasse Aqueous Extract (SBAE) [J].

Arora, Neha ;

Patel, Alok ;

Pruthi, Parul A. ;

Pruthi, Vikas .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2016, 180 (01) :109-121

[3] Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production [J].

Arora, Neha ;

Patel, Alok ;

Pruthi, Parul A. ;

Pruthi, Vikas .

BIORESOURCE TECHNOLOGY, 2016, 213 :79-87

[4]

Beacham T A, 2015, Biotechnol Rep (Amst), V7, P87, DOI 10.1016/j.btre.2015.05.007

[5]

Bligh E, 1959, CAN J BIOCH, V37

[6] Light-harvesting complex II (LHCII) and its supramolecular organization in Chlamydomonas reinhardtii [J].

Drop, Bartlomiej ;

Webber-Birungi, Mariam ;

Yadav, Sathish K. N. ;

Filipowicz-Szymanska, Alicja ;

Fusetti, Fabrizia ;

Boekema, Egbert J. ;

Croce, Roberta .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2014, 1837 (01) :63-72

[7] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8] Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors [J].

Fan, Jianhua ;

Cui, Yanbin ;

Wan, Minxi ;

Wang, Weiliang ;

Li, Yuanguang .

BIOTECHNOLOGY FOR BIOFUELS, 2014, 7

[9] The Starch Granule-Associated Protein EARLY STARVATION1 Is Required for the Control of Starch Degradation in Arabidopsis thaliana Leaves [J].

Feike, Doreen ;

Seung, David ;

Graf, Alexander ;

Bischof, Sylvain ;

Ellick, Tamaryn ;

Coiro, Mario ;

Soyk, Sebastian ;

Eicke, Simona ;

Mettler-Altmann, Tabea ;

Lu, Kuan Jen ;

Trick, Martin ;

Zeeman, Samuel C. ;

Smith, Alison M. .

PLANT CELL, 2016, 28 (06) :1472-1489

[10] Occurrence of copper resistant mutants in the toxic cyanobacteria Microcystis aeruginosa:: characterisation and future implications in the use of copper sulphate as algaecide [J].

García-Villada, L ;

Rico, M ;

Altamirano, M ;

Sánchez-Martín, L ;

López-Rodas, V ;

Costas, E .

WATER RESEARCH, 2004, 38 (08) :2207-2213

← 1 2 3 4 →