Down-Regulation of ID2-AS1 Alleviates the Neuronal Injury Induced by 1-Methy1-4-Phenylpyridinium in Human Neuroblastoma Cell Line SH-SY5Y Cells Through Regulating miR-199a-5p/IFNAR1/JAK2/STAT1 Axis

被引：9
作者：
Xu, Furong [1 ]
Wang, Hui [2 ]
Tian, Ju [3 ]
Xu, Haiyan [4 ]
机构：
[1] Chengdu Seventh Peoples Hosp, Dept Neurol, Chengdu 610000, Sichuan, Peoples R China
[2] Liaocheng Peoples Hosp, Dept Neurol, Liaocheng 252000, Shandong, Peoples R China
[3] Qingdao 8th Peoples Hosp, Dept Funct Inspect, Qingdao 266000, Shandong, Peoples R China
[4] Huazhong Univ Sci & Technol, Tongji Med Coll, Puai Hosp, Dept Gastroenterol,Wuhan Fourth Hosp, 473 Han Zheng St, Wuhan 430033, Hubei, Peoples R China
来源：
NEUROCHEMICAL RESEARCH | 2021年 / 46卷 / 08期
关键词：
Parkinson's disease; Neuronal injury; ID2-AS1; miR-199a-5p; IFNAR1; JAK2; STAT1; JAK/STAT SIGNALING PATHWAY; NONCODING RNAS; PARKINSONS-DISEASE; MECHANISMS; APOPTOSIS; STRESS; CANCER; STAT3;
D O I：
10.1007/s11064-021-03356-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We aimed to illustrate the roles and molecular mechanisms of ID2-AS1 in parkinson's disease (PD). Methods: qRT-PCR detected the expression of ID2-AS1. CCK-8, LDH release assays the effect of ID2-AS1 knockdown on PD cells. Flow cytometry and Western Blot were used to detect the effect of ID2-AS1 inhibition on PD cell apoptosis. ELISA analysis showed that ID2-AS1 inhibition can reduce the inflammation of PD cells. ROS activity assay showed that inhibiting ID2-AS1 attenuated the oxidative stress induced by 1-methy1-4-phenylpyridinium (MPP+). RNA binding protein immunoprecipitation assay showed that ID2-AS1 is mainly located in the cytoplasm. The luciferase reporter assay is used to verify the interaction. In our study, ID2-AS1 was concentration-dependently and time-dependently up-regulated in MPP+ -treated human neuroblastoma cell line SH-SY5Y. ID2-AS1 knockdown enhanced cell proliferation and decreased cell death in PD cells. Knockdown of ID2-AS1 attenuates MPP+ -induced cytotoxicity in SH-SY5Y cells. ID2-AS1 is a sponge of miR-199a-5p. IFNAR1 is a target of miR-199a-5p. Inhibition of miR-199a-5p and overexpression of IFNAR1 alleviate the inhibitory effect of ID2-AS1 knockdown on MPP+ triggered neuronal injury. Inhibition of miR-199a-5p and overexpression of IFNAR1 alleviate the inhibitory effect of ID2-AS1 knockdown on MPP+ -triggered JAK2/STAT1 activation. Overall, down-regulation of ID2-AS1 alleviated the neuronal injury in PD through regulating miR-199a-5p/IFNAR1/JAK2/STAT1 axis.
引用
收藏
页码：2192 / 2203
页数：12
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