Identification of wild hosts of tomato yellow leaf curl virus in South-Eastern Iran

被引：0

作者：

Shamshiri, Marzieh ^{[1
]}

Heydarnejad, Jahangir ^{[1
]}

Kamali, Mehdi ^{[1
]}

Pouramini, Najmeh ^{[1
]}

Massumi, Hossain ^{[1
]}

机构：

[1] Shahid Bahonar Univ Kerman, Dept Plant Protect, Coll Agr, Kerman 7616914111, Iran

来源：

ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION | 2019年 / 52卷 / 9-10期

关键词：

Tomato yellow leaf curl virus; rolling circle amplification; wild hosts; Physalis divaricata L; Ammi majus L; Hibiscus trionum L; POLYMERASE-CHAIN-REACTION; NATURAL OCCURRENCE; GENETIC DIVERSITY; PALAMPUR VIRUS; BEGOMOVIRUS; WEEDS; GEMINIVIRUS; SEQUENCE; DISEASE; PLANTS;

D O I：

10.1080/03235408.2019.1682231

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In this research, to identify natural wild hosts of TYLCV, 44 symptomatic samples were collected in or around harvested tomato fields in south-eastern Iran and tested for the TYLCV infection by PCR. Among four PCR-positive plant species, full-length genome of TYLCV was amplified by rolling circle amplification (RCA) method only from a ground cherry (Physalis divaricata L., Solanaceae) sample. Cloning and full-length genome sequencing of a ground cherry isolate (G4) showed that it comprises 2756 nucleotides (nts) in length and shares 87.7%-95.2% nt sequence identities with TYLCV isolates reported from Iran, Oman and Pakistan. The G4 isolate of TYLCV was successfully transmitted to tomato plants and the original host (P. divaricata) via agroinoculation and showed typical symptoms. According to the results of this research, four symptomatic weed species appear to be alternative hosts of TYLCV and play the role of the primary inoculum for the viral infection.

引用

页码：917 / 929

页数：13

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