The utility of MASS-FIX to detect and monitor monoclonal proteins in the clinic

被引:101
作者
Milani, Paolo [1 ,2 ,3 ]
Murray, David L. [4 ]
Barnidge, David R. [4 ]
Kohlhagen, Mindy C. [4 ]
Mills, John R. [4 ]
Merlini, Giampaolo [2 ]
Dasari, Surendra [5 ]
Dispenzieri, Angela [1 ]
机构
[1] Mayo Clin, Div Hematol, 200 First St SW, Rochester, MN 55905 USA
[2] Univ Pavia, Dept Mol Med, Amyloidosis Res & Treatment Ctr, Pavia, Italy
[3] Univ Pavia, PhD Program Expt Med, Pavia, Italy
[4] Mayo Clin, Dept Lab Med, Rochester, MN USA
[5] Mayo Clin, Dept Hlth Sci Res, Rochester, MN USA
关键词
IMMUNOGLOBULIN LIGHT-CHAINS; SERUM; SPECTROMETRY; MYELOMA; IMMUNOFIXATION; DIAGNOSIS; AL;
D O I
10.1002/ajh.24772
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The detection and quantification of monoclonal-proteins (M-proteins) are necessary for the diagnosis and evaluation of response in plasma cell dyscrasias. Immunoglobulin enrichment-coupled with matrix-assisted laser desorption ionization time-of-flight mass-spectrometry (MASS-FIX) is a simple and inexpensive method to identify M-proteins, but its clinical generalizability has not yet been elucidated. We compared MASS-FIX to protein electrophoresis (PEL), serum/urine immunofixation-electrophoresis (IFE), and quantitative serum free-light chain (FLC) for the identification of M-proteins in different clinical diagnoses. Paired serum and urine samples from 257 patients were tested. There were six patients for whom s-IFE and FLC ratio were positive and serum MASS-FIX was negative, but when serum and urine MASS-FIX results were combined, only one patient with light chain-MGUS was missed. Serum/urine-MASS-FIX detected M-proteins in 18 patients with negative serum/urine-PEL/IFE and serum-FLC, 10 of whom had multiple myeloma or AL amyloidosis, who were mistakenly thought to have complete hematologic response by serum/urine-PEL/IFE and serum-FLC. Nearly half of the AL amyloidosis patients had atypical spectra, which may prove to be a clue to the diagnosis and pathogenesis of the disease. In conclusion, MASS-FIX has a comparable sensitivity with PEL/IFE/FLC methods and can help inform the clinical diagnosis.
引用
收藏
页码:772 / 779
页数:8
相关论文
共 26 条
[1]  
AGUZZI F, 1992, EUR J HAEMATOL, V48, P192
[2]   Monitoring free light chains in serum using mass spectrometry [J].
Barnidge, David R. ;
Dispenzieri, Angela ;
Merlini, Giampaolo ;
Katzmann, Jerry A. ;
Murray, David L. .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2016, 54 (06) :1073-1083
[3]   Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to detect monoclonal immunoglobulin light chains in serum and urine [J].
Barnidge, David R. ;
Krick, Thomas P. ;
Griffin, Timothy J. ;
Murray, David L. .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2015, 29 (21) :2057-2060
[4]   Using Mass Spectrometry to Monitor Monoclonal Immunoglobulins in Patients with a Monoclonal Gammopathy [J].
Barnidge, David R. ;
Dasari, Surendra ;
Botz, Chad M. ;
Murray, Danelle H. ;
Snyder, Melissa R. ;
Katzmann, Jerry A. ;
Dispenzieri, Angela ;
Murray, David L. .
JOURNAL OF PROTEOME RESEARCH, 2014, 13 (03) :1419-1427
[5]   Clonotypic Light Chain Peptides Identified for Monitoring Minimal Residual Disease in Multiple Myeloma without Bone Marrow Aspiration [J].
Bergen, H. Robert, III ;
Dasari, Surendra ;
Dispenzieri, Angela ;
Mills, John R. ;
Ramirez-Alvarado, Marina ;
Tschumper, Renee C. ;
Jelinek, Diane F. ;
Barnidge, David R. ;
Murray, David L. .
CLINICAL CHEMISTRY, 2016, 62 (01) :243-251
[6]   Detecting monoclonal light chains in urine: microLC-ESI-Q-TOF mass spectrometry compared to immunofixation electrophoresis [J].
Botz, Chad M. ;
Barnidge, David R. ;
Murray, David L. ;
Katzmann, Jerry A. .
BRITISH JOURNAL OF HAEMATOLOGY, 2014, 167 (03) :437-438
[7]   Reliable typing of systemic amyloidoses through proteomic analysis of subcutaneous adipose tissue [J].
Brambilla, Francesca ;
Lavatelli, Francesca ;
Di Silvestre, Dario ;
Valentini, Veronica ;
Rossi, Rossana ;
Palladini, Giovanni ;
Obici, Laura ;
Verga, Laura ;
Mauri, Pierluigi ;
Merlini, Giampaolo .
BLOOD, 2012, 119 (08) :1844-1847
[8]   Heterogeneity in primary structure, post-translational modifications, and germline gene usage of nine full-length amyloidogenic κ1 immunoglobulin light chains [J].
Connors, Lawreen H. ;
Jiang, Yan ;
Budnik, Marianna ;
Theberge, Roger ;
Prokaeva, Tatiana ;
Bodi, Kip L. ;
Seldin, David C. ;
Costello, Catherine E. ;
Skinner, Martha .
BIOCHEMISTRY, 2007, 46 (49) :14259-14271
[9]   A practical approach to the diagnosis of systemic amyloidoses [J].
de Larrea, Carlos Fernandez ;
Verga, Laura ;
Morbini, Patrizia ;
Klersy, Catherine ;
Lavatelli, Francesca ;
Foli, Andrea ;
Obici, Laura ;
Milani, Paolo ;
Capello, Gian Luca ;
Paulli, Marco ;
Palladini, Giovanni ;
Merlini, Giampaolo .
BLOOD, 2015, 125 (14) :2239-2244
[10]   Serum free light chains, not urine specimens, should be used to evaluate response in light-chain multiple myeloma [J].
Dejoie, Thomas ;
Corre, Jill ;
Caillon, Helene ;
Hulin, Cyrille ;
Perrot, Aurore ;
Caillot, Denis ;
Boyle, Eileen ;
Chretien, Marie-Lorraine ;
Fontan, Jean ;
Belhadj, Karim ;
Brechignac, Sabine ;
Decaux, Olivier ;
Voillat, Laurent ;
Rodon, Philippe ;
Fitoussi, Olivier ;
Araujo, Carla ;
Benboubker, Lotfi ;
Fontan, Charlotte ;
Tiab, Mourad ;
Godmer, Pascal ;
Luycx, Odile ;
Allangba, Olivier ;
Pignon, Jean-Michel ;
Fuzibet, Jean-Gabriel ;
Legros, Laurence ;
Stoppa, Anne Marie ;
Dib, Mamoun ;
Pegourie, Brigitte ;
Orsini-Piocelle, Frederique ;
Karlin, Lionel ;
Arnulf, Bertrand ;
Roussel, Murielle ;
Garderet, Laurent ;
Mohty, Mohamad ;
Meuleman, Nathalie ;
Doyen, Chantal ;
Lenain, Pascal ;
Macro, Margaret ;
Leleu, Xavier ;
Facon, Thierry ;
Moreau, Philippe ;
Attal, Michel ;
Avet-Loiseau, Herve .
BLOOD, 2016, 128 (25) :2941-2948