Chemical dynamics in proteins: The photoisomerization of retinal in bacteriorhodopsin

被引：230

作者：

Gai, F ^{[1
]}

Hasson, KC ^{[1
]}

McDonald, JC ^{[1
]}

Anfinrud, PA ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

SCIENCE | 1998年 / 279卷 / 5358期

关键词：

D O I：

10.1126/science.279.5358.1886

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chemical dynamics in proteins are discussed, with bacteriorhodopsin serving as a model system. Ultrafast time-resolved methods used to probe the chemical dynamics of retinal photoisomerization in bacteriorhodopsin are discussed, along with future prospects for ultrafast time-resolved crystallography, The photoisomerization of retinal in bacteriorhodopsin is far more selective and efficient than in solution, the origins of which are discussed in the context of a three-state model for the photoisomerization reaction coordinate. The chemical dynamics are complex, with the excited-state relaxation exhibiting a multiexponential decay with well-defined rate constants, Possible origins for the two major components are also discussed.

引用

页码：1886 / 1891

页数：6

共 69 条

[1] PICOSECOND KINETICS OF FLUORESCENCE FROM CHROMOPHORE OF PURPLE MEMBRANE-PROTEIN OF HALOBACTERIUM-HALOBIUM [J].

ALFANO, RR ;

GOVINDJEE, WYR ;

BECHER, B ;

EBREY, TG .

BIOPHYSICAL JOURNAL, 1976, 16 (05) :541-545

[2]

Althaus T, 1995, ISRAEL J CHEM, V35, P227

[3] THE INITIAL REACTION DYNAMICS OF THE LIGHT-DRIVEN CHLORIDE PUMP HALORHODOPSIN [J].

ARLT, T ;

SCHMIDT, S ;

ZINTH, W ;

HAUPTS, U ;

OESTERHELT, D .

CHEMICAL PHYSICS LETTERS, 1995, 241 (5-6) :559-565

[4] PICOSECOND TIME-RESOLVED RESONANCE RAMAN-SPECTROSCOPY OF THE INITIAL TRANS TO CIS ISOMERIZATION IN THE BACTERIORHODOPSIN PHOTOCYCLE [J].

ATKINSON, GH ;

BRACK, TL ;

BLANCHARD, D ;

RUMBLES, G .

CHEMICAL PHYSICS, 1989, 131 (01) :1-15

[5] SOLVENT VISCOSITY AND PROTEIN DYNAMICS [J].

BEECE, D ;

EISENSTEIN, L ;

FRAUENFELDER, H ;

GOOD, D ;

MARDEN, MC ;

REINISCH, L ;

REYNOLDS, AH ;

SORENSEN, LB ;

YUE, KT .

BIOCHEMISTRY, 1980, 19 (23) :5147-5157

[6] REVISED ASSIGNMENT OF ENERGY-STORAGE IN THE PRIMARY PHOTOCHEMICAL EVENT IN BACTERIORHODOPSIN [J].

BIRGE, RR ;

COOPER, TM ;

LAWRENCE, AF ;

MASTHAY, MB ;

ZHANG, CF ;

ZIDOVETZKI, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (11) :4327-4328

[7] PROTEIN-BASED COMPUTERS [J].

BIRGE, RR .

SCIENTIFIC AMERICAN, 1995, 272 (03) :90-95

[8] VIBRATIONALLY EXCITED RETINAL IN THE BACTERIORHODOPSIN PHOTOCYCLE - PICOSECOND TIME-RESOLVED ANTI-STOKES RESONANCE RAMAN-SCATTERING [J].

BRACK, TL ;

ATKINSON, GH .

JOURNAL OF PHYSICAL CHEMISTRY, 1991, 95 (06) :2351-2356

[9] RESONANCE RAMAN-SPECTRA OF BACTERIORHODOPSINS PRIMARY PHOTOPRODUCT - EVIDENCE FOR A DISTORTED 13-CIS RETINAL CHROMOPHORE [J].

BRAIMAN, M ;

MATHIES, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :403-407

[10] VIBRATIONAL SPECTROSCOPY OF BACTERIORHODOPSIN MUTANTS - LIGHT-DRIVEN PROTON TRANSPORT INVOLVES PROTONATION CHANGES OF ASPARTIC-ACID RESIDUE-85, RESIDUE-96, AND RESIDUE-212 [J].

BRAIMAN, MS ;

MOGI, T ;

MARTI, T ;

STERN, LJ ;

KHORANA, HG ;

ROTHSCHILD, KJ .

BIOCHEMISTRY, 1988, 27 (23) :8516-8520

← 1 2 3 4 5 6 7 →