Nanoparticle Aggregate-Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA

被引：6

作者：

Annink, Carla ^{[1
]}

Gill, Ron ^{[1
]}

机构：

[1] Univ Twente, Nanobiophys Grp, MIRA Inst Biomed Engn & Tech Med, NL-7500 AE Enschede, Netherlands

来源：

PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION | 2014年 / 31卷 / 09期

关键词：

MICROSCOPY; SCATTERING; RESONANCE;

D O I：

10.1002/ppsc.201400061

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

(Graph Presented) Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface-enhanced fluorescence (SEF)-based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV). © 2014 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：943 / 947

页数：5

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