Utilizing simple biochemical measurements to predict lifetime output of biocatalysts in continuous isothermal processes

被引:78
作者
Rogers, Thomas A. [1 ,3 ]
Bommarius, Andreas S. [1 ,2 ,3 ]
机构
[1] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
[2] Georgia Inst Technol, Sch Chem Biochem, Atlanta, GA 30332 USA
[3] Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA
关键词
Biocatalysis; Biochemical engineering; Catalyst deactivation; Stability; Total turnover number; Catalyst lifetime productivity; TEM-1; BETA-LACTAMASE; GLUCOSE-DEHYDROGENASE; SULFOLOBUS-SOLFATARICUS; THERMAL INACTIVATION; MEMBRANE REACTOR; STABILITY; ENZYME; TEMPERATURE; KINETICS; PATHWAY;
D O I
10.1016/j.ces.2009.12.005
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The expected product yield of a biocatalyst during its useful lifetime is an important consideration when designing a continuous biocatalytic process. One important indicator of lifetime biocatalyst productivity is the dimensionless total turnover number (TTN). Here, a method is proposed for estimating the TTN of a given biocatalyst from readily measured biochemical quantities, namely the specific activity and the deactivation half-life, measured under identical conditions. We demonstrate that this method may be applied to any enzyme whose thermal deactivation follows first-order kinetics, regardless of the number of unfolding intermediates, and that the TTN method circumvents the potential problems associated with measuring specific catalyst output when a portion of the enzyme is already unfolded. The TTN estimation was applied to several representative biocatalysts to demonstrate its applicability in identifying the most cost-effective catalyst from a pool of engineered mutants with similar activity and thermal stability. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2118 / 2124
页数:7
相关论文
共 33 条
  • [21] Recent applications of biocatalysis in developing green chemistry for chemical synthesis at the industrial scale
    Ran, Ningqing
    Zhao, Lishan
    Chen, Zhenming
    Tao, Junhua
    [J]. GREEN CHEMISTRY, 2008, 10 (04) : 361 - 372
  • [22] Biocatalysis: Towards ever greener biodiesel production
    Robles-Medina, A.
    Gonzalez-Moreno, P. A.
    Esteban-Cerdan, L.
    Molina-Grima, E.
    [J]. BIOTECHNOLOGY ADVANCES, 2009, 27 (04) : 398 - 408
  • [23] Deactivation of TEM-1 β-Lactamase Investigated by Isothermal Batch and Non-Isothermal Continuous Enzyme Membrane Reactor Methods
    Rogers, Thomas A.
    Daniel, Roy M.
    Bommarius, Andreas S.
    [J]. CHEMCATCHEM, 2009, 1 (01) : 131 - 137
  • [24] Recent advances in developing chemoenzymatic processes for active pharmaceutical ingredients
    Tao, Junhua
    Zhao, Lishan
    Ran, Ningqing
    [J]. ORGANIC PROCESS RESEARCH & DEVELOPMENT, 2007, 11 (02) : 259 - 267
  • [25] Tramper J, 1996, BIOTECHNOL BIOENG, V52, P290, DOI 10.1002/(SICI)1097-0290(19961020)52:2<290::AID-BIT8>3.0.CO
  • [26] 2-L
  • [27] INVESTIGATION OF THE FOLDING PATHWAY OF THE TEM-1 BETA-LACTAMASE
    VANHOVE, M
    RAQUET, X
    FRERE, JM
    [J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1995, 22 (02) : 110 - 118
  • [28] Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media
    Vazquez-Figueroa, E.
    Yeh, V.
    Broering, J. M.
    Chaparro-Riggers, J. F.
    Bommarius, A. S.
    [J]. PROTEIN ENGINEERING DESIGN & SELECTION, 2008, 21 (11) : 673 - 680
  • [29] Development of a thermostable glucose dehydrogenase by a structure-guided consensus concept
    Vazquez-Figueroa, Eduardo
    Chaparro-Riggers, Javier
    Bommarius, Andreas S.
    [J]. CHEMBIOCHEM, 2007, 8 (18) : 2295 - 2301
  • [30] Hyperthermophilic enzymes: Sources, uses, and molecular mechanisms for thermostability
    Vieille, C
    Zeikus, GJ
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (01) : 1 - +