The expected product yield of a biocatalyst during its useful lifetime is an important consideration when designing a continuous biocatalytic process. One important indicator of lifetime biocatalyst productivity is the dimensionless total turnover number (TTN). Here, a method is proposed for estimating the TTN of a given biocatalyst from readily measured biochemical quantities, namely the specific activity and the deactivation half-life, measured under identical conditions. We demonstrate that this method may be applied to any enzyme whose thermal deactivation follows first-order kinetics, regardless of the number of unfolding intermediates, and that the TTN method circumvents the potential problems associated with measuring specific catalyst output when a portion of the enzyme is already unfolded. The TTN estimation was applied to several representative biocatalysts to demonstrate its applicability in identifying the most cost-effective catalyst from a pool of engineered mutants with similar activity and thermal stability. (C) 2009 Elsevier Ltd. All rights reserved.
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Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Vazquez-Figueroa, E.
Yeh, V.
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Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biochem, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Yeh, V.
Broering, J. M.
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Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Stanford Univ, Dept Bioengn, Clark Ctr, Stanford, CA 94305 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Broering, J. M.
Chaparro-Riggers, J. F.
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Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Chaparro-Riggers, J. F.
Bommarius, A. S.
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Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biochem, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
机构:
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Vazquez-Figueroa, E.
Yeh, V.
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h-index: 0
机构:
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biochem, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Yeh, V.
Broering, J. M.
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h-index: 0
机构:
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Stanford Univ, Dept Bioengn, Clark Ctr, Stanford, CA 94305 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Broering, J. M.
Chaparro-Riggers, J. F.
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h-index: 0
机构:
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Chaparro-Riggers, J. F.
Bommarius, A. S.
论文数: 0引用数: 0
h-index: 0
机构:
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
Parker H Petit Inst Bioengn & Biosci, Sch Chem & Biochem, Atlanta, GA 30332 USAParker H Petit Inst Bioengn & Biosci, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA