Effects on Growth of Human Osteoblast-Like Cells of Three Nonsteroidal Anti-Inflammatory Drugs: Metamizole, Dexketoprofen, and Ketorolac

被引:20
作者
De Luna-Bertos, Elvira [1 ]
Ramos-Torrecillas, Javier [1 ]
Manzano-Moreno, Francisco Javier [1 ]
Garcia-Martinez, Olga [1 ]
Ruiz, Concepcion [1 ]
机构
[1] Univ Granada, Sch Hlth Sci, Inst Neurosci, Dept Nursing, Granada 18971, Spain
关键词
NSAIDs; osteoblasts; MG63; bone tissue; cell growth; PHAGOCYTIC CAPACITY; BONE; DIFFERENTIATION; PROLIFERATION; DICLOFENAC; IBUPROFEN; NSAIDS; ACETAMINOPHEN; CYCLE;
D O I
10.1177/1099800414527155
中图分类号
R47 [护理学];
学科分类号
1011 ;
摘要
Some nonsteroidal anti-inflammatory drugs (NSAIDs) have adverse effects on bone tissue. The objective of this study was to determine the effect of different doses of dexketoprofen, ketorolac, and metamizole on growth of the osteoblast MG63 cell line. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide spectrophotometry results showed that MG63 cell growth was significantly inhibited after 24 hr of culture with doses of 10, 20, 100, or 1,000 mu M of each NSAID and with doses of 0.1, 1, or 5 mu M of dexketoprofen and ketorolac but not metamizole. Cell-cycle studies revealed that dexketoprofen and ketorolac treatments significantly arrested the cell cycle in phase G(0)/G(1), increasing the percentage of cells in this phase. Apoptosis/necrosis studies showed significant changes versus control cells, with an increased percentage of cells in apoptosis after treatment with 10, 100, or 1,000 mu M of metamizole and after treatment with 1, 10, 100, or 1,000 mu M of dexketoprofen or ketorolac. In conclusion, treatment of osteoblast-like cells with high doses of the NSAIDs tested increased not only the percentage of cells in apoptosis but also the percentage of necrotic cells.
引用
收藏
页码:62 / 67
页数:6
相关论文
共 34 条
[1]   Effect of Ibuprofen on Osteoblast Differentiation of Porcine Bone Marrow-Derived Progenitor Cells [J].
Abukawa, Harutsugi ;
Phelps, Maynard ;
Jackson, Pamela ;
Smith, R. Malcolm ;
Vacanti, Joseph P. ;
Kaban, Leonard B. ;
Troulis, Maria J. .
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2009, 67 (11) :2412-2417
[2]  
Arpornmaeklong P, 2008, INT J ORAL MAX IMPL, V23, P1071
[3]   Influence of diclofenac (group of nonsteroidal anti-inflammatory drugs) on fracture healing [J].
Beck, A ;
Krischak, G ;
Sorg, T ;
Augat, P ;
Farker, K ;
Merkel, U ;
Kinzl, L ;
Claes, L .
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY, 2003, 123 (07) :327-332
[4]   KETOROLAC - A REVIEW OF ITS PHARMACODYNAMIC AND PHARMACOKINETIC PROPERTIES, AND THERAPEUTIC POTENTIAL [J].
BUCKLEY, MMT ;
BROGDEN, RN .
DRUGS, 1990, 39 (01) :86-109
[5]   Anti-inflammatory drugs suppress proliferation and induce apoptosis through altering expressions of cell cycle regulators and pro-apoptotic factors in cultured human osteoblasts [J].
Chang, Je-Ken ;
Li, Ching-Ju ;
Liao, Hsiu-Jun ;
Wang, Chih-Kuang ;
Wang, Gwo-Jaw ;
Ho, Mei-Ling .
TOXICOLOGY, 2009, 258 (2-3) :148-156
[6]   Nonsteroidal anti-inflammatory drug effects on osteoblastic cell cycle, cytotoxicity, and cell death [J].
Chang, JK ;
Wang, GJ ;
Tsai, ST ;
Ho, ML .
CONNECTIVE TISSUE RESEARCH, 2005, 46 (4-5) :200-210
[7]   A turbulent decade for NSAIDs: update on current concepts of classification, epidemiology, comparative efficacy, and toxicity [J].
Conaghan, Philip G. .
RHEUMATOLOGY INTERNATIONAL, 2012, 32 (06) :1491-1502
[8]   Effect of Aspirin on Cell Growth of Human MG-63 Osteosarcoma Line [J].
De Luna-Bertos, E. ;
Ramos-Torrecillas, J. ;
Garcia-Martinez, O. ;
Diaz-Rodriguez, L. ;
Ruiz, C. .
SCIENTIFIC WORLD JOURNAL, 2012,
[9]   Antigenic Phenotype and Phagocytic Capacity of MG-63 Osteosarcoma Line [J].
Diaz-Rodriguez, L. ;
Garcia-Martinez, O. ;
Arroyo-Morales, M. ;
Reyes-Botella, C. ;
Ruiz, C. .
INTEGRATIVE PHYSIOLOGY, 2009, 1173 (S1) :E46-E54
[10]  
Diaz-Rodriguez L., 2012, ADV SCI LETT, V17, P325, DOI [10.1166/asl.2012.4272, DOI 10.1166/asl.2012.4272]