Blood virosphere in febrile Tanzanian children

被引：18

作者：

Cordey, Samuel ^{[1
,2
,3
,4
]}

Laubscher, Florian ^{[1
,2
,3
,4
,5
]}

Hartley, Mary-Anne ^{[6
]}

Junier, Thomas ^{[7
,8
]}

Keitel, Kristina ^{[9
,10
]}

Docquier, Mylene ^{[11
,12
]}

Guex, Nicolas ^{[2
,13
,14
]}

Iseli, Christian ^{[13
,14
]}

Vieille, Gael ^{[1
,3
,4
]}

Le Mercier, Philippe ^{[15
]}

Gleizes, Anne ^{[15
]}

Samaka, Josephine ^{[16
]}

Mlaganile, Tarsis ^{[16
]}

Kagoro, Frank ^{[16
]}

Masimba, John ^{[16
]}

Said, Zamzam ^{[16
]}

Temba, Hosiana ^{[16
]}

Elbanna, Gasser H. ^{[6
]}

Tapparel, Caroline ^{[17
]}

Zanella, Marie-Celine ^{[2
,3
,4
]}

Xenarios, Ioannis ^{[18
,19
]}

Fellay, Jacques ^{[7
,8
,20
,21
]}

D'Acremont, Valerie ^{[5
,9
]}

Kaiser, Laurent ^{[1
,2
,3
,4
,22
]}

机构：

[1] Geneva Univ Hosp, Div Infect Dis, Geneva, Switzerland

[2] Univ Geneva, Lab Virol, Div Infect Dis, Geneva, Switzerland

[3] Univ Geneva, Div Lab Med, Univ Hosp Geneva, Geneva, Switzerland

[4] Univ Geneva, Fac Med, Geneva, Switzerland

[5] Univ Lausanne, Ctr Primary Care & Publ Hlth Unisante, Lausanne, Switzerland

[6] Ecole Polytech Fed Lausanne, Machine Learning & Optimizat Lab, Intelligent Global Hlth, Lausanne, Switzerland

[7] Ecole Polytech Fed Lausanne, Global Hlth Inst, Sch Life Sci, Lausanne, Switzerland

[8] SIB Swiss Inst Bioinformat, Lausanne, Switzerland

[9] Univ Basel, Swiss Trop & Publ Hlth Inst, Basel, Switzerland

[10] Univ Bern, Univ Hosp Bern, Dept Pediat, Dept Paediat Emergency Med,Inselspital, Bern, Switzerland

[11] Univ Geneva, iGE3 Genom Platform, Geneva, Switzerland

[12] Univ Geneva, Dept Genet & Evolut, Geneva, Switzerland

[13] Univ Lausanne, Bioinformat Competence Ctr, Lausanne, Switzerland

[14] Ecole Polytech Fed Lausanne, Lausanne, Switzerland

[15] SIB Swiss Inst Bioinformat, SwissProt Grp, Geneva, Switzerland

[16] Ifakara Hlth Inst, Dar Es Salaam, Tanzania

[17] Univ Geneva Med Sch, Dept Microbiol & Mol Med, Geneva, Switzerland

[18] Health2030 Genome Ctr, Geneva, Switzerland

[19] Univ Lausanne, Agora Ctr, Lausanne, Switzerland

[20] Univ Lausanne Hosp, Precis Med Unit, Lausanne, Switzerland

[21] Univ Lausanne, Lausanne, Switzerland

[22] Univ Hosp Geneva, Geneva Ctr Emerging Viral Dis, Geneva, Switzerland

来源：

EMERGING MICROBES & INFECTIONS | 2021年 / 10卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

Blood virome; virosphere; metagenomic next-generation sequencing; fever; children; HUMAN ANELLOVIRUSES; ACUTE GASTROENTERITIS; HUMAN VIROME; METAGENOMICS; DISCOVERY; MOSQUITOS; VIRUSES; STOOL; CELL; RNA;

D O I：

10.1080/22221751.2021.1925161

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Viral infections are the leading cause of childhood acute febrile illnesses motivating consultation in sub-Saharan Africa. The majority of causal viruses are never identified in low-resource clinical settings as such testing is either not part of routine screening or available diagnostic tools have limited ability to detect new/unexpected viral variants. An indepth exploration of the blood virome is therefore necessary to clarify the potential viral origin of fever in children. Metagenomic next-generation sequencing is a powerful tool for such broad investigations, allowing the detection of RNA and DNA viral genomes. Here, we describe the blood virome of 816 febrile children (<5 years) presenting at outpatient departments in Dar es Salaam over one-year. We show that half of the patients (394/816) had at least one detected virus recognized as causes of human infection/disease (13.8% enteroviruses (enterovirus A, B, C, and rhinovirus A and C), 12% rotaviruses, 11% human herpesvirus type 6). Additionally, we report the detection of a large number of viruses (related to arthropod, vertebrate or mammalian viral species) not yet known to cause human infection/disease, highlighting those who should be on the radar, deserve specific attention in the febrile paediatric population and, more broadly, for surveillance of emerging pathogens.

引用

页码：982 / 993

页数：12

共 51 条

[31] The blood DNA virome in 8,000 humans [J].