Restriction fragment length polymorphism analysis of open reading frame 5 gene of porcine reproductive and respiratory syndrome virus isolates in Korea - Brief report

被引：22
作者：
Cheon, DS
Chae, C [1 ]
机构：
[1] Seoul Natl Univ, Coll Vet Med, Dept Vet Pathol, Suwon 441744, Kyounggi Do, South Korea
[2] Seoul Natl Univ, Sch Agr Biotechnol, Suwon 441744, Kyounggi Do, South Korea
来源：
ARCHIVES OF VIROLOGY | 2000年 / 145卷 / 07期
关键词：
D O I：
10.1007/s007050070105
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The genetic variability of porcine reproductive and respiratory syndrome virus (PRRSV) was studied by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR)-amplified fragments among 50 Korean isolates from open reading frame 5. All Korean PRRSVs were isolated from the field cases after the marketing of an U.S. ATCC VR2332-derived modified live PRRSV vaccine. Combining the restriction enzyme digestion patterns obtained with MluI, HincII, SacII, and HaeIII, we observed 19 distinct RFLP patterns. Seventeen out of 50 PRRSV isolates (34%) exhibited the modified live PRRSV vaccine RFLP pattern. The genomic variations that have been identified in the present study seemed to represent characteristic features of the Korean PRRSV isolates. PCR-based RFLP analysis using several restriction enzymes provides a good genetic estimate for isolate differentiation.
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页码：1481 / 1488
页数：8
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