Krapfen/dMyd88 is required for the establishment of dorsoventral pattern in the Drosophila embryo

被引：38

作者：

Charatsi, I ^{[1
]}

Luschnig, S ^{[1
]}

Bartoszewski, S ^{[1
]}

Nüsslein-Volhard, C ^{[1
]}

Moussian, B ^{[1
]}

机构：

[1] Max Planck Inst Entwicklungsbiol, Genet Abt, D-72076 Tubingen, Germany

来源：

MECHANISMS OF DEVELOPMENT | 2003年 / 120卷 / 02期

关键词：

Drosophila; development; dorsoventral axis; pattern formation; axis specification; tube; pelle; MyD88; toll; torso;

D O I：

10.1016/S0925-4773(02)00410-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Drosophila, the dorsoventral axis is set up by the action of the dorsal group of genes and cactus, which have been ordered genetically in a linear pathway. We have identified and characterised krapfen (kra) as a new member of the dorsal-group genes. kra encodes for the Drosophila homologue of MyD88, an adapter protein operating in the mammalian IL-1 pathway. Epistasis experiments reveal that kra acts between the receptor Toll and the cytoplasmic factor Tube. We show that there is a direct interaction between Kra and Tube presumably mediated by the death domains present in both proteins. Tube in turn interacts with its downstream effector Pelle through death domain association. We therefore suggest that upon Toll activation, Kra associates with Pelle and Tube, in an heterotrimeric complex. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：219 / 226

页数：8

共 43 条

[21] THE DNA-BINDING SUBUNIT OF NF-KAPPA-B IS IDENTICAL TO FACTOR KBF1 AND HOMOLOGOUS TO THE REL ONCOGENE PRODUCT [J].

KIERAN, M ;

BLANK, V ;

LOGEAT, F ;

VANDEKERCKHOVE, J ;

LOTTSPEICH, F ;

LEBAIL, O ;

URBAN, MB ;

KOURILSKY, P ;

BAEUERLE, PA ;

ISRAEL, A .

CELL, 1990, 62 (05) :1007-1018

[22] FUNCTION OF TORSO IN DETERMINING THE TERMINAL ANLAGEN OF THE DROSOPHILA EMBRYO [J].

KLINGLER, M ;

ERDELYI, M ;

SZABAD, J ;

NUSSLEINVOLHARD, C .

NATURE, 1988, 335 (6187) :275-277

[23] The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults [J].

Lemaitre, B ;

Nicolas, E ;

Michaut, L ;

Reichhart, JM ;

Hoffmann, JA .

CELL, 1996, 86 (06) :973-983

[24] Drosophila host defense:: Differential induction of antimicrobial peptide genes after infection by various classes of microorganisms [J].

Lemaitre, B ;

Reichhart, JM ;

Hoffmann, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) :14614-14619

[25] GENETIC AND MOLECULAR CHARACTERIZATION OF TUBE, A DROSOPHILA GENE MATERNALLY REQUIRED FOR EMBRYONIC DORSOVENTRAL POLARITY [J].

LETSOU, A ;

ALEXANDER, S ;

ORTH, K ;

WASSERMAN, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (03) :810-814

[26]

Luscombe NM, 2000, GENOME BIOL, V1

[27] THE SPATZLE GENE ENCODES A COMPONENT OF THE EXTRACELLULAR SIGNALING PATHWAY ESTABLISHING THE DORSAL-VENTRAL PATTERN OF THE DROSOPHILA EMBRYO [J].

MORISATO, D ;

ANDERSON, KV .

CELL, 1994, 76 (04) :677-688

[28]

RAY RP, 1991, DEVELOPMENT, V113, P35

[29] A GRADIENT OF NUCLEAR-LOCALIZATION OF THE DORSAL PROTEIN DETERMINES DORSOVENTRAL PATTERN IN THE DROSOPHILA EMBRYO [J].

ROTH, S ;

STEIN, D ;

NUSSLEINVOLHARD, C .

CELL, 1989, 59 (06) :1189-1202

[30]

Sambrook J., 1989, MOL CLONING

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