Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline

被引:4
作者
Cerella, Claudia
Mearelli, Cristina
De Nicola, Milena
D'Alessio, Maria
Magrini, Andrea
Bergamaschi, Antonio
Ghibelli, Lina
机构
[1] Univ Roma Tor Vergata, Dipartimento Biol, I-00133 Rome, Italy
[2] Univ Roma Tor Vergata, Cattedra Med Lavoro, I-00133 Rome, Italy
[3] Univ Cattolica Sacro Cuore, Cattedra Med Lavoro, I-00168 Rome, Italy
来源
SODIUM-CALCIUM EXCHANGE AND THE PLASMA MEMBRANE CA2+-ATPASE IN CELL FUNCTION: FIFTH INTERNATIONAL CONFERENCE | 2007年 / 1099卷
关键词
apoptosis; endoplasmic reticulum; calcium; thapsigargin; chlortetracycline;
D O I
10.1196/annals.1387.021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.
引用
收藏
页码:490 / 493
页数:4
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