Citrobacter rodentium mouse model of bacterial infection

被引：72

作者：

Crepin, Valerie F. ^{[1
]}

Collins, James W. ^{[1
]}

Habibzay, Maryam ^{[1
]}

Frankel, Gad ^{[1
]}

机构：

[1] Imperial Coll, Dept Life Sci, MRC Ctr Mol Bacteriol & Infect, London, England

来源：

NATURE PROTOCOLS | 2016年 / 11卷 / 10期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1038/nprot.2016.100

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Infection of mice with Citrobacter rodentium is a robust model to study bacterial pathogenesis, mucosal immunology, the health benefits of probiotics and the role of the microbiota during infection. C. rodentium was first isolated by Barthold from an outbreak of mouse diarrhea in Yale University in 1972 and was 'rediscovered' by Falkow and Schauer in 1993. Since then the use of the model has proliferated, and it is now the gold standard for studying virulence of the closely related human pathogens enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively). Here we provide a detailed protocol for various applications of the model, including bacterial growth, site-directed mutagenesis, mouse inoculation (from cultured cells and after cohabitation), monitoring of bacterial colonization, tissue extraction and analysis, immune responses, probiotic treatment and microbiota analysis. The main protocol, from mouse infection to clearance and analysis of tissues and host responses, takes similar to 5 weeks to complete.

引用

页码：1851 / 1876

页数：26

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