RELATIONSHIP BETWEEN SERUM MIR-182 EXPRESSION LEVEL AND PATHOLOGICAL GRADE IN GLIOMA AND ITS VALUE IN EARLY DIAGNOSIS OF GLIOMA AND PROGNOSIS

<bold>Objective</bold>: To analyse the relationship between the serum miR-182 expression level and pathological grade of glioma and the value of miR-182 in the early diagnosis of, and prognosis for, glioma. <bold>Methods:</bold> From January 2019 to January 2020, 125 glioma patients treated in our hospital were selected as an observation group, while 40 healthy people in our hospital were selected as a control group during the same period. Using the recommended WHO grades, the patients in the observation group were divided into a low-grade group (n =67) and high-grade group (n=58) and also divided into a good prognosis group (n=79) and poor prognosis group (n=46). Ten ml of fasting venous blood was extracted from all subjects, with total RNA extracted via an miR-VPARISA kit, and total RNA concentration and purity of total RNA detected via a nucleic acid quantitative detector. The levels of miR-182 and miR-124 expression were detected via a TaqqManmicroRNA reverse transcription kit from Ambion (USA). Pearson correlations were used to analyse the relationships between miR-182, miR-124 expression level and pathological grade, and ROC curves were used to examine the value of miR-182 and miR-124 in the early diagnosis of, and prognosis for, glioma. <bold> </bold>Results: The levels of miR-182 and miR-124 in the observation group were significantly higher than those in the control group (P<0.01). In addition, the levels of miR-182 and miR-124 in patients with glioma in the high-grade group were significantly higher than those in the patients in the low-grade group (P<0.01). Also, the levels of miR-182 and miR-124 in glioma patients with a poor prognosis were significantly higher than those in patients with a good prognosis (P<0.01). According to the Pearson correlation analysis, the expression levels of miR-182 and miR-124 are positively correlated with the WHO classification (r=0.451, 0.561, P<0.05). According to the ROC curve analysis, the AUC for the early diagnosis of glioma with miR-182 was 0.836, the sensitivity was 86.79%, and the specificity was 82.46%, whereas the AUC for the early diagnosis of glioma with miR-124 was 0.715, the sensitivity was 73.48%, and the specificity was 72.16%.The AUC for the prognosis of glioma patients assessed via miR-182 was 0.849, the sensitivity was 82.79%, and the specificity was 82.48%. The AUC, sensitivity, and specificity of miR-124 for prognosis were 0.774, 79.15%, and 76.15%, respectively. <bold> </bold>Conclusion: The serum levels of miR-182 and miR-124 in glioma patients are highly expressed and increase with the increase in the pathological grade of the patients, which is of certain value in the early diagnosis and evaluation of the prognosis of patients. Of the two, miR-182 has higher value and can be widely used in clinical practice.