Note: Dynamic point spread function for single and multiphoton fluorescence microscopy

被引：5

作者：

Mondal, Partha Pratim ^{[1
]}

Mandal, Subhra ^{[2
]}

Diaspro, Alberto ^{[3
]}

机构：

[1] Indian Inst Sci, Dept Instrumentat, Bangalore 560012, Karnataka, India

[2] SISSA, I-34149 Trieste, Italy

[3] Italian Inst Technol, I-16163 Genoa, Italy

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2010年 / 81卷 / 04期

关键词：

bioluminescence; biomedical optical imaging; fluorescence; molecular biophysics; optical microscopy; optical transfer function; ENDOPLASMIC-RETICULUM; 2-PHOTON EXCITATION; FIELD; DIFFRACTION; RESOLUTION; TRAFFICKING; IMPROVEMENT; RECEPTOR; LIMIT;

D O I：

10.1063/1.3378683

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We propose and demonstrate a dynamic point spread function (PSF) for single and multiphoton fluorescence microscopy. The goal is to generate a PSF whose shape and size can be maneuvered from highly localized to elongated one, thereby allowing shallow-to-depth excitation capability during active imaging. The PSF is obtained by utilizing specially designed spatial filter and dynamically altering the filter parameters. We predict potential applications in nanobioimaging and fluorescence microscopy.

引用

页数：3

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