Androgen deprivation therapy has no effect on Pim-1 expression in a mouse model of prostate cancer

被引:5
作者
Wang, Jiang [1 ,2 ]
Li, Gang [1 ]
Li, Bo [1 ]
Song, Hualin [1 ]
Shang, Zhiqun [1 ]
Jiang, Ning [1 ]
Niu, Yuanjie [1 ]
机构
[1] Tianjin Med Univ, Tianjin Inst Urol, Dept Urol, Hosp 2, 23 Ping Jiang Rd, Tianjin 300211, Peoples R China
[2] Tianjin Municipal Res Inst Family Planning, Tianjin 300131, Peoples R China
关键词
androgen-dependent prostate cancer; androgen deprivation therapy; androgen independent prostate cancer; Pim-1; animal model; orthotopic implanting; LNCaP cell; androgen receptor; TUMOR-GROWTH; KINASE; RECEPTOR; PHOSPHORYLATION; OVEREXPRESSION; PROGRESSION; DOCETAXEL; SURVIVAL; ROLES;
D O I
10.3892/ol.2017.6010
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The aim of the present study was to observe the dynamic changes of proto-oncogene, serine/threonine kinase, Pim-1 at the gene and protein level in a mouse model of prostate cancer following surgical castration. Using LNCaP cells to establish a subcutaneous xenograft model and orthotopic prostate cancer BALB/c nude mouse models, the xenograft models were divided into an androgen-dependent prostate cancer group (ADPC), an androgen deprivation therapy (ADT) group and an androgen independent prostate cancer (AIPC) group. Reverse transcription-polymerase chain reaction (RT-PCR), RT-quantitative PCR, ELISA and immunohistochemistry analyses were performed to compare the expression levels of Pim-1, prostate-specific antigen (PSA) and androgen receptor (AR) in tumor tissue of three subgroups. Agarose gel electrophoresis revealed that the RT-PCR results of the ADPC (0.59 +/- 0.01) and AIPC groups (1.14 +/- 0.015) were significantly different when compared with the ADT group (0.62 +/- 0.026; P < 0.05). As for RT-qPCR, the Delta Cq of Pim-1 in the ADPC (6.15 +/- 0.34) and AIPC (4.56 +/- 0.23) groups were significantly different compared with the ADT group (5.11 +/- 0.21; P < 0.05). Using 2-(Delta Delta Cq) as a relative quantification method to analyze the data, the amplification products of Pim-1 increased by 2.05 and 3.01 times in the ADT and AIPC groups, respectively. ELISA demonstrated the following: The serum concentration of PSA was 0 ng/ml in the control group, 0.48 +/- 0.025 ng/ml in the ADPC group and 0.87 +/- 0.023 ng/ml in the AIPC group, which were significantly different compared with the ADT group (0.17 +/- 0.032 ng/ml; P < 0.01). Upon immunohistochemical staining, the protein expression levels of Pim-1 and AR, respectively, were 0.017 +/- 0.0021 and 0.032 +/- 0.009 in the ADPC group, 0.024 +/- 0.0019 and 0.040 +/- 0.011 in the AIPC group, and 0.018 +/- 0.0013 and 0.019 +/- 0.006 in the ADT group. The protein levels of Pim-1 and AR in the ADPC and AIPC groups were significantly different compared with the ADT group (P < 0.01). In addition, an orthotopic prostate cancer animal model of ADT was successfully established in the current study, and further investigation revealed that ADT did not affect the expression of Pim-1 at the gene or protein levels; thus, it is hypothesized that Pim-1 may be important in the proliferation and differentiation of prostate cancer during ADT.
引用
收藏
页码:4364 / 4370
页数:7
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