COMPARISON OF RESAZURIN AND MTT METHODS ON STUDIES OF CITOTOXICITY IN HUMAN TUMOR CELL LINES

被引：0

作者：

Escobar M, Linamaria ^{[1
]}

Rivera, Augusto ^{[2
]}

Aristizabal G, Fabio A. ^{[1
]}

机构：

[1] Univ Nacl Colombia, Fac Ciencias, Dept Farm, Grp Farmacogenet Canc, Bogota 14490, Colombia

[2] Univ Nacl Colombia, Fac Ciencias, Dept Quim, Bogota 14490, Colombia

来源：

VITAE-REVISTA DE LA FACULTAD DE QUIMICA FARMACEUTICA | 2010年 / 17卷 / 01期

关键词：

Doxorubicin; MTT; resazurine; cellular viability; ALAMAR-BLUE; IN-VITRO; CYTOTOXICITY TESTS; MICROTITER ASSAY; GROWTH; PROLIFERATION; MITOCHONDRIAL; VIABILITY; CANCER;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A study was carried comparing the resazurina and MTT methods as indicators of indirect measure of cellular survival applied to valuated cytotoxic activity in order to assess the suitability of each of the two detection systems. The comparison was made through the valuation of cytotoxicity on three cell lines derived from human tumors: HEp-2, HT-29 and HeLa, evaluating three synthetic compounds during a treatment period of 48 hours using as a reference activity Doxorubicin HCl. The results obtained by the two methods showed a similar performance under the conditions tested in cell lines.

引用

页码：67 / 74

页数：8

共 50 条

[21] Halofuginone enhances the radiation sensitivity of human tumor cell lines [J].

Cook, John A. ;

Choudhuri, Rajani ;

DeGraff, William ;

Gamson, Janet ;

Mitchell, James B. .

CANCER LETTERS, 2010, 289 (01) :119-126

[22] Comparison of Global DNA Methylation Patterns in Human Melanoma Tissues and Their Derivative Cell Lines [J].

Rodger, Euan J. ;

Almomani, Suzan N. ;

Ludgate, Jackie L. ;

Stockwell, Peter A. ;

Baguley, Bruce C. ;

Eccles, Michael R. ;

Chatterjee, Aniruddha .

CANCERS, 2021, 13 (09)

[23] A comparison of in vitro methods for determining the membrane receptor expression in cell lines [J].

Novy, Zbynek ;

Barta, Pavel ;

Mandikova, Jana ;

Laznicek, Milan ;

Trejtnar, Frantisek .

NUCLEAR MEDICINE AND BIOLOGY, 2012, 39 (07) :893-896

[24] Concentration of endogenous estrogens and estrogen metabolites in the NCI-60 human tumor cell lines [J].

Xu, Xia ;

Veenstra, Timothy D. .

GENOME MEDICINE, 2012, 4

[25] Quantification of integrin subunits on human prostatic cell lines - Comparison of nontumorigenic and tumorigenic lines [J].

HaywoodReid, PL ;

Zipf, DR ;

Springer, WR .

PROSTATE, 1997, 31 (01) :1-8

[26] Rapid in vitro chemosensitivity analysis of human colon tumor cell lines [J].

Waldenmaier, DS ;

Babarina, A ;

Kischkel, FC .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2003, 192 (03) :237-245

[27] Functional activity of human sodium/iodide symporter in tumor cell lines [J].

Petrich, I ;

Knapp, WH ;

Pötter, E .

NUKLEARMEDIZIN-NUCLEAR MEDICINE, 2003, 42 (01) :15-18

[28] Zoledronic Acid as an Antimetastatic Agent for Different Human Tumor Cell Lines [J].

Muinelo-Romay, Laura ;

Garcia, Daniel ;

Alonso-Alconada, Lorena ;

Vieito, Maria ;

Carmona, Marta ;

Martinez, Nieves ;

Aguin, Santiago ;

Abal, Miguel ;

Lopez-Lopez, Rafael .

ANTICANCER RESEARCH, 2013, 33 (12) :5295-5300

[29] Comparison of cancer stem cell antigen expression by tumor cell lines and by tumor biopsies from dogs with melanoma and osteosarcoma [J].

Guth, Amanda M. ;

Deogracias, Mike ;

Dow, Steven W. .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2014, 161 (3-4) :132-140

[30] Comparison of hypocrellin B-mediated sonodynamic responsiveness between sensitive and multidrug-resistant human gastric cancer cell lines [J].

Liu, Yichen ;

Bai, Hong ;

Wang, Haiping ;

Wang, Xiaobing ;

Liu, Quanhong ;

Zhang, Kun ;

Wang, Pan .

JOURNAL OF MEDICAL ULTRASONICS, 2019, 46 (01) :15-26

← 1 2 3 4 5 →