Thymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms

被引：5

作者：

Almeida, L. ^{[1
]}

Lopes, N. ^{[1
,2
]}

Gaio, V. ^{[1
,2
]}

Cavaleiro, C. ^{[3
,4
]}

Salgueiro, L. ^{[3
,4
]}

Silva, V. ^{[5
,6
,7
]}

Poeta, P. ^{[5
,6
,7
]}

Cerca, N. ^{[1
,2
]}

机构：

[1] Univ Minho, Ctr Biol Engn CEB, Lab Res Biofilms Rosario Oliveira LIBRO, Campus Gualtar, P-4710057 Braga, Portugal

[2] LABBELS Associate Lab, Braga, Portugal

[3] Univ Coimbra, Fac Pharm, Coimbra, Portugal

[4] Univ Coimbra, Fac Sci & Technol, Dept Chem Engn, CIEPQPF, Coimbra, Portugal

[5] Univ Tras Os Montes & Alto Douro UTAD, Dept Vet Sci, Microbiol & Antibiot Resistance Team MicroART, Vila Real, Portugal

[6] Univ Tras Os Montes & Alto Douro UTAD, Associate Lab Anim & Vet Sci AL4AnimalS, Vet & Anim Res Ctr, Vila Real, Portugal

[7] Univ Nova Lisboa, Associated Lab Green Chem LAQV REQUIMTE, Lisbon, Portugal

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2022年 / 74卷 / 05期

关键词：

biofilm biomass; cell culturability; cellular activity; EO; minimum inhibitory concentration; minimum lethal concentration; MRSA; XTT; COAGULASE-NEGATIVE STAPHYLOCOCCI; CHEMICAL-COMPOSITION; ANTIBIOTIC SUSCEPTIBILITY; PORTUGUESE THYMBRA; INFECTIONS; QUANTIFICATION; CARVACROL; COMPONENT;

D O I：

10.1111/lam.13665

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T. capitata EO was first investigated on a group of clinical isolates from septicaemias, diabetic foot ulcers and osteomyelitis. Biofilms were incubated with the EO at the MLC and its anti-biofilm potential was investigated. A strong antimicrobial activity was observed, with MIC and MLC values between 0 center dot 32 and 0 center dot 64 mg l(-1). However, the concentration of EO necessary for the eradication of planktonic cells was insufficient to significantly reduce the biofilm biomass of some isolates. Nevertheless, cell culturability and overall cellular metabolism was strongly reduced in all biofilms tested, only when the EO was tested. Contrary to the tested antibiotics, T. capitata EO showed a significant antimicrobial activity against MRSA biofilms, by reducing cellular metabolism and cellular culturability.

引用

页码：787 / 795

页数：9

共 56 条

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