Xylanase Production by Penicillium citrinum in Laboratory-scale Stirred Tank Reactor

被引：12

作者：

Ghoshal, G. ^{[1
]}

Banerjee, U. C. ^{[2
]}

Shivhare, U. S. ^{[1
]}

机构：

[1] Panjab Univ, Dr SS Bhatnagar Univ Inst Chem Engn & Technol, Chandigarh 160014, India

[2] Natl Inst Pharmaceut Educ & Res, Dept Pharmaceut Technol, Sas Nagar 160062, Punjab, India

来源：

CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY | 2014年 / 28卷 / 03期

关键词：

xylanase; Penicillium citrinum; sugarcane bagasse; volumetric mass transfer coefficient; kinetics models; TRICHODERMA-REESEI; THERMOMYCES-LANUGINOSUS; AGITATION RATE; CELLULASE; OPTIMIZATION; AERATION; GROWTH; FERMENTATION; BIOREACTOR; COMPONENTS;

D O I：

10.15255/CABEQ.2013.1885

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Xylanase constitutes an important class of hydrolases, and is used in numerous industrial applications. The aim of the present work was to study the production of xylanase from Penicillium citrinum MTCC 9620 in a 5 L stirred tank bioreactor. Effect of various process parameters; pH, temperature, aeration, agitation rates, substrate concentration, and, dissolved oxygen (DO) concentration on xylanase production were studied. Combination of all the optimized parameters resulted in 2.5 times higher enzyme activity as compared to the shake flask fermentation after 96 h. Effect of varying agitation and aeration on the volumetric mass transfer coefficient (K(L)a) was determined. It revealed that K(L)a is influenced by both aeration and agitation. Growth kinetics of P. citrinum MTCC 9620 in bioreactor was studied using Monod, Moser, Contois and Edward equation. Based on R-2, SE and pattern of residuals, the microbial growth kinetics of R citrinum MTCC 9620 was effectively represented by Moser equation.

引用

页码：399 / 408

页数：10

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