Enhanced nisin production by increasing genes involved in nisin Z biosynthesis in Lactococcus lactis subsp lactis A164

被引：44

作者：

Cheigh, CI

Park, H

Choi, HJ

Pyun, YR ^{[1
]}

机构：

[1] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[2] Sunmoon Univ, Div Appl Biol Sci, Chungnam 336708, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 03期

关键词：

enhanced production; Lactococcus lactis; nisin; nisin gene cluster;

D O I：

10.1007/s10529-004-7661-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nisin Z production in Lactococcus lactis subsp. lactis A164 was improved by introducing multicopy genes, nisZ, nisRK, or nisFEG, involved in nisin biosynthesis into A164 strain. A similar growth profile was obtained from all strains tested. However, the cells expressing nisRK produced 25,000 AU ml(-1) by compared to 16,000 AU ml(-1) by the control strain. Northern blot analysis revealed that over-expression of nisRK promoted the transcription of the nisZ gene. The A164 strain expressing multicopy nisFEG also had an increased nisin Z production (25,000 AU ml(-1)) but produced the nisin more slowly than the cells expressing multicopy nisRK.

引用

页码：155 / 160

页数：6

共 16 条

[1] Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp lactis A164 using expanded bed ion exchange chromatography [J].