Arduino Due based tool to facilitate in vivo two-photon excitation microscopy

被引：6

作者：

Artoni, Pietro ^{[1
]}

Landi, Silvia

Sato, Sebastian Sulis

Luin, Stefano

Ratto, Gian Michele

机构：

[1] CNR, Ist Nanosci, NEST, I-56127 Pisa, Italy

来源：

BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 04期

关键词：

LOW-COST; MICROCONTROLLER; TISSUES; SENSOR; SYSTEM; PROBE; IONS;

D O I：

10.1364/BOE.7.001604

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-photon excitation spectroscopy is a powerful technique for the characterization of the optical properties of genetically encoded and synthetic fluorescent molecules. Excitation spectroscopy requires tuning the wavelength of the Ti: sapphire laser while carefully monitoring the delivered power. To assist laser tuning and the control of delivered power, we developed an Arduino Due based tool for the automatic acquisition of high quality spectra. This tool is portable, fast, affordable and precise. It allowed studying the impact of scattering and of blood absorption on two-photon excitation light. In this way, we determined the wavelength-dependent deformation of excitation spectra occurring in deep tissues in vivo. (C) 2016 Optical Society of America

引用

页码：1604 / 1613

页数：10

共 20 条

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[2]

Artoni P., 2016, SPECTRUMINO ARDUINO

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