Arduino Due based tool to facilitate in vivo two-photon excitation microscopy

被引：6
作者：
Artoni, Pietro [1 ]
Landi, Silvia
Sato, Sebastian Sulis
Luin, Stefano
Ratto, Gian Michele
机构：
[1] CNR, Ist Nanosci, NEST, I-56127 Pisa, Italy
来源：
BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 04期
关键词：
LOW-COST; MICROCONTROLLER; TISSUES; SENSOR; SYSTEM; PROBE; IONS;
D O I：
10.1364/BOE.7.001604
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two-photon excitation spectroscopy is a powerful technique for the characterization of the optical properties of genetically encoded and synthetic fluorescent molecules. Excitation spectroscopy requires tuning the wavelength of the Ti: sapphire laser while carefully monitoring the delivered power. To assist laser tuning and the control of delivered power, we developed an Arduino Due based tool for the automatic acquisition of high quality spectra. This tool is portable, fast, affordable and precise. It allowed studying the impact of scattering and of blood absorption on two-photon excitation light. In this way, we determined the wavelength-dependent deformation of excitation spectra occurring in deep tissues in vivo. (C) 2016 Optical Society of America
引用
收藏
页码：1604 / 1613
页数：10
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