Gregatins, a Group of Related Fungal Secondary Metabolites, Inhibit Aspects of Quorum Sensing in Gram-Negative Bacteria

被引:6
作者
Beenker, Wouter A. G. [1 ,2 ]
Hoeksma, Jelmer [1 ,2 ]
den Hertog, Jeroen [1 ,2 ,3 ]
机构
[1] Hubrecht Inst KNAW, Utrecht, Netherlands
[2] Univ Med Ctr Utrecht, Utrecht, Netherlands
[3] Leiden Univ, Inst Biol Leiden, Leiden, Netherlands
关键词
quorum sensing; gregatins; Chromobacterium violaceum; Pseudomonas aeruginosa; antimicrobial activity; fungi; PSEUDOMONAS-AERUGINOSA VIRULENCE; DNA RELEASE; IDENTIFICATION; GENES; MOLECULE; INDOLE; AJOENE;
D O I
10.3389/fmicb.2022.934235
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Quorum sensing (QS) is a process that regulates gene expression based on cell density. In bacteria, QS facilitates collaboration and controls a large number of pathways, including biofilm formation and virulence factor production, which lead to lower sensitivity to antibiotics and higher toxicity in the host, respectively. Inhibition of QS is a promising strategy to combat bacterial infections. In this study, we tested the potential of secondary metabolites from fungi to inhibit bacterial QS using a library derived from more than ten thousand different fungal strains. We used the reporter bacterium, Chromobacterium violaceum, and identified 39 fungal strains that produced QS inhibitor activity. These strains expressed two QS inhibitors that had been described before and eight QS inhibitors that had not been described before. Further testing for QS inhibitor activity against the opportunistic pathogen Pseudomonas aeruginosa led to the identification of gregatins as an interesting family of compounds with QS inhibitor activity. Although various gregatins inhibited QS in P. aeruginosa, these gregatins did not inhibit virulence factor production and biofilm formation. We conclude that gregatins inhibit some, but not all aspects of QS.
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收藏
页数:15
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