Response to drought and salt stress in leaves of poplar (Populus alba x Populus glandulosa): Expression profiling by oligonucleotide microarray analysis

被引：31

作者：

Yoon, Seo-Kyung ^{[1
,3
]}

Park, Eung-Jun ^{[1
]}

Choi, Young-Im ^{[1
]}

Bae, Eun-Kyung ^{[1
]}

Kim, Joon-Hyeok ^{[1
]}

Park, So-Young ^{[2
]}

Kang, Kyu-Suk ^{[3
]}

Lee, Hyoshin ^{[1
]}

机构：

[1] Korea Forest Res Inst, Dept Forest Genet Resources, Suwon 441847, South Korea

[2] Chungbuk Natl Univ, Dept Hort Sci, Cheongju 361763, South Korea

[3] Seoul Natl Univ, Dept Forest Sci, Seoul 151742, South Korea

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2014年 / 84卷

关键词：

Drought stress; Gene expression; Oligonucleotide microarray; Populus; Salt stress; SUPPRESSION SUBTRACTIVE HYBRIDIZATION; TRANSCRIPTION FACTORS; GENE-EXPRESSION; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; DESERT POPLAR; HIGH-SALINITY; TOLERANCE; COLD; IDENTIFICATION;

D O I：

10.1016/j.plaphy.2014.09.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Drought and salt stresses are major environmental constraints on forest productivity. To identify genes responsible for stress tolerance, we conducted a genome-wide analysis in poplar (Populus alba x Populus glandulosa) leaves exposed to drought and salt (NaCl) stresses. We investigated gene expression at the mRNA level using oligonucleotide microarrays containing 44,718 genes from Populus trichocarpa. A total of 1604 and 1042 genes were up-regulated (>= 2-fold; P value < 0.05) by drought and salt stresses, respectively, and 765 genes were up-regulated by both stresses. In addition, 2742 and 1685 genes were down-regulated by drought and salt stresses, respectively, and 1564 genes were down-regulated by both stresses. The large number of genes regulated by both stresses suggests that crosstalk occurs between the drought and salt stress responses. Most up-regulated genes were involved in functions such as subcellular localization, signal transduction, metabolism, and transcription. Among the up-regulated genes, we identified 47 signaling proteins, 65 transcription factors, and 43 abiotic stress-related genes. Several genes were modulated by only one of the two stresses. About 25% of the genes significantly regulated by these stresses are of unknown function, suggesting that poplar may provide an opportunity to discover novel stress-related genes. (C) 2014 Elsevier Masson SAS. All rights reserved.

引用

页码：158 / 168

页数：11

共 64 条

[1] Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba x Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl [J].

Bae, Eun-Kyung ;

Lee, Hyoshin ;

Lee, Jae-Soon ;

Noh, Eun-Woon ;

Choi, Young-Im ;

Lee, Byung-Hyun ;

Choi, Dong-Woog .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2012, 58 :151-158

[2] Isolation and characterization of osmotic stress-induced genes in poplar cells by suppression subtractive hybridization and cDNA microarray analysis [J].

Bae, Eun-Kyung ;

Lee, Hyoshin ;

Lee, Jae-Soon ;

Noh, Eun-Woon .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2010, 48 (2-3) :136-141

[3] Linking the Salt Transcriptome with Physiological Responses of a Salt-Resistant Populus Species as a Strategy to Identify Genes Important for Stress Acclimation [J].

Brinker, Monika ;

Brosche, Mikael ;

Vinocur, Basia ;

Abo-Ogiala, Atef ;

Fayyaz, Payam ;

Janz, Dennis ;

Ottow, Eric A. ;

Cullmann, Andreas D. ;

Saborowski, Joachim ;

Kangasjarvi, Jaakko ;

Altman, Arie ;

Polle, Andrea .

PLANT PHYSIOLOGY, 2010, 154 (04) :1697-1709

[4] Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert -: art. no. R101 [J].

Brosché, M ;

Vinocur, B ;

Alatalo, ER ;

Lamminmäki, A ;

Teichmann, T ;

Ottow, EA ;

Djilianov, D ;

Afif, D ;

Bogeat-Triboulot, MB ;

Altman, A ;

Polle, A ;

Dreyer, E ;

Rudd, S ;

Lars, P ;

Auvinen, P ;

Kangasjärvi, J .

GENOME BIOLOGY, 2005, 6 (12)

[5] Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves [J].

Caruso, Aurore ;

Chefdor, Francoise ;

Carpin, Sabine ;

Depierreux, Christiane ;

Delmotte, Francis M. ;

Kahlem, Guy ;

Morabito, Domenico .

JOURNAL OF PLANT PHYSIOLOGY, 2008, 165 (09) :932-941

[6] Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell [J].

Chaves, M. M. ;

Flexas, J. ;

Pinheiro, C. .

ANNALS OF BOTANY, 2009, 103 (04) :551-560

[7] The role of WRKY transcription factors in plant abiotic stresses [J].

Chen, Ligang ;

Song, Yu ;

Li, Shujia ;

Zhang, Liping ;

Zou, Changsong ;

Yu, Diqiu .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2012, 1819 (02) :120-128

[8] The salt-responsive transcriptome of Populus simonii x Populus nigra via DGE [J].

Chen, Su ;

Jiang, Jing ;

Li, Huiyu ;

Liu, Guifeng .

GENE, 2012, 504 (02) :203-212

[9] Wheat LEA genes, PMA80 and PMA1959, enhance dehydration tolerance of transgenic rice (Oryza sativa L.) [J].

Cheng, ZQ ;

Targolli, J ;

Huang, XQ ;

Wu, R .

MOLECULAR BREEDING, 2002, 10 (1-2) :71-82

[10] Characterization of five novel dehydration-responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum [J].

Deng, X ;

Phillips, J ;

Meijer, AH ;

Salamini, F ;

Bartels, D .

PLANT MOLECULAR BIOLOGY, 2002, 49 (06) :601-610

← 1 2 3 4 5 6 7 →