On the fundamental imaging-depth limit in two-photon microscopy

被引：6

作者：

Theer, P ^{[1
]}

Denk, W ^{[1
]}

机构：

[1] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

来源：

FEMTOSECOND LASER APPLICATIONS IN BIOLOGY | 2004年 / 5463卷

关键词：

two-photon microscopy; imaging-depth limit; turbid media;

D O I：

10.1117/12.548057

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The limit on imaging-depth in two-photon microscopy depends on parameters of the imaging system such as available power, wavelength, numerical aperture, and fluorescencecollection field-of-view and on properties of the sample such as scattering and absorption cross-sections and fluorophore distribution. These dependencies are discussed and strategies for optimizing the imaging depth are presented.

引用

收藏

页码：45 / 55

页数：11

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