Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation

被引：25
作者：
Wang, H. H. [1 ,2 ,3 ]
Cui, Q. [3 ]
Zhang, T. [2 ,3 ]
Wang, Z. B. [2 ]
Ouyang, Y. C. [2 ]
Shen, W. [3 ]
Ma, J. Y. [3 ]
Schatten, H. [4 ]
Sun, Q. Y. [1 ,2 ,3 ]
机构：
[1] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
[2] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China
[3] Qingdao Agr Univ, Shandong Univ, Coll Anim Sci & Technol, Inst Reprod Sci,Key Lab Anim Reprod & Germplasm E, Qingdao 266109, Peoples R China
[4] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA
来源：
HISTOCHEMISTRY AND CELL BIOLOGY | 2016年 / 145卷 / 06期
关键词：
Meiotic maturation; Cortical granules; Rab3A; Rab27A; Rab35; CORTICAL GRANULES; MAMMALIAN EGGS; CAENORHABDITIS-ELEGANS; ASYMMETRIC DIVISION; SECRETORY GRANULES; PORCINE OOCYTE; CELL-DIVISION; IN-VITRO; ACTIN; FERTILIZATION;
D O I：
10.1007/s00418-015-1404-5
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation. The three Rab family members showed different localization patterns in oocytes. Microinjection of siRNA, antibody injection, or inhibitor treatment showed that (1) Rab3A regulates peripheral spindle and cortical granule (CG) migration, polarity establishment, and asymmetric division; (2) Rab27A regulates CG exocytosis following MII-stage oocyte activation; and (3) Rab35 plays an important role in spindle organization and morphology maintenance, and thus meiotic nuclear maturation. These results show that Rab proteins play important roles in mouse oocyte meiotic maturation and activation and that different members exert different distinct functions.
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收藏
页码：647 / 657
页数：11
相关论文
共 54 条
[1]   Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis [J].
Bustos, Matias A. ;
Lucchesi, Ornella ;
Ruete, Maria C. ;
Mayorga, Luis S. ;
Tomes, Claudia N. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (30) :E2057-E2066
[2]   A soft cortex is essential for asymmetric spindle positioning in mouse oocytes [J].
Chaigne, Agathe ;
Campillo, Clement ;
Gov, Nir S. ;
Voituriez, Raphael ;
Azoury, Jessica ;
Umana-Diaz, Claudia ;
Almonacid, Maria ;
Queguiner, Isabelle ;
Nassoy, Pierre ;
Sykes, Cecile ;
Verlhac, Marie-Helene ;
Terret, Marie-Emilie .
NATURE CELL BIOLOGY, 2013, 15 (08) :958-U163
[3]   TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation [J].
Chen, Lei ;
Ge, Zhao-Jia ;
Wang, Zhen-Bo ;
Sun, Tianyi ;
Ouyang, Ying-Chun ;
Sun, Qing-Yuan ;
Sun, Ying-Pu .
CELL CYCLE, 2014, 13 (17) :2723-2732
[4]   An ARF6/Rab35 GTPase Cascade for Endocytic Recycling and Successful Cytokinesis [J].
Chesneau, Laurent ;
Dambournet, Daphne ;
Machicoane, Mickael ;
Kouranti, Ilektra ;
Fukuda, Mitsunori ;
Goud, Bruno ;
Echard, Arnaud .
CURRENT BIOLOGY, 2012, 22 (02) :147-153
[5]   Rab35 regulates neurite outgrowth and cell shape [J].
Chevallier, Julien ;
Koop, Charles ;
Srivastava, Archana ;
Petrie, Ryan J. ;
Lamarche-Vane, Nathalie ;
Presley, John F. .
FEBS LETTERS, 2009, 583 (07) :1096-1101
[6]   Rab35-A vesicular traffic-regulating small GTPase with actin modulating roles [J].
Chua, Christelle En Lin ;
Lim, Yi Shan ;
Tang, Bor Luen .
FEBS LETTERS, 2010, 584 (01) :1-6
[7]   A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes [J].
Clift, Dean ;
Schuh, Melina .
NATURE COMMUNICATIONS, 2015, 6
[8]  
CRAN DG, 1990, J REPROD FERTIL, P177
[9]   FREE CA-2+ INCREASES IN EXPONENTIAL PHASES DURING MOUSE OOCYTE ACTIVATION [J].
CUTHBERTSON, KSR ;
WHITTINGHAM, DG ;
COBBOLD, PH .
NATURE, 1981, 294 (5843) :754-757
[10]   Rab27A and its effector MyRIP link secretory granules to F-actin and control their motion towards release sites [J].
Desnos, C ;
Schonn, JS ;
Huet, S ;
Tran, VS ;
El-Amraoui, A ;
Raposo, GA ;
Fanget, I ;
Chapuis, C ;
Ménasché, G ;
de saint Basile, G ;
Petit, C ;
Cribier, S ;
Henry, JP ;
Darchen, F .
JOURNAL OF CELL BIOLOGY, 2003, 163 (03) :559-570
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