Comparative analysis of HBV basic core promoter/pre-core gene mutations and viral quasispecies diversity in HIV/HBV co-infected and HBV mono-infected patients

被引：1

作者：

Deng, Haohui ^{[1
,2
,3
]}

Gao, Hongbo ^{[3
]}

Liu, Yu ^{[1
,2
]}

Xu, Ying ^{[1
,2
]}

Yang, Juncheng ^{[1
,2
]}

Zhao, Miaoxian ^{[1
,2
]}

Liu, Huiyuan ^{[3
]}

Wang, Zhanhui ^{[1
,2
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, State Key Lab Organ Failure Res, Guangdong Prov Key Lab Viral Hepatitis Res,Dept I, Guangzhou, Peoples R China

[2] Southern Med Univ, Nanfang Hosp, Hepatol Unit, Guangzhou, Peoples R China

[3] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Infect Dis Ctr, Guangzhou, Peoples R China

来源：

ACTA VIROLOGICA | 2022年 / 66卷 / 01期

基金：

中国国家自然科学基金;

关键词：

human immunodeficiency virus; hepatitis B virus; mutations; viral quasispecies; next-generation sequencing; HEPATITIS-B-VIRUS; COINFECTION; HIV; RESISTANCE;

D O I：

10.4149/av_2022_103

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human immunodeficiency virus (HIV)/hepatitis B virus (HBV) co-infection accelerates the progression of HBV-related liver diseases. HBV basic core promoter (BCP)/pre-core (preC) gene mutations may be one of the most important risk factors. In this study, a total of 230 patients were recruited, and 199 patients whose HBV BCP/preC gene were successfully amplified and sequenced, including 99 HIV/HBV co-infected and 100 HBV mono-infected patients. Next-generation sequencing was used for detection of BCP/preC mutations which were then compared in patients with different HBV genotypes and different HBeAg statuses, and 1% and 20% cutoff values were defined to evaluate the mutations. HBV quasispecies diversity was also compared in HIV/HBV co-infected and HBV mono-infected patients. Among the patients infected with HBV genotype C and HBeAg-negative status, the frequency of A1762T/G1764A double mutations was significantly lower in HIV/HBV co-infected patients than in HBV mono-infected patients (53.3% vs. 100.0%, P = 0.008) regardless of the 1% or 20% cutoff value level. However, A1762T/G1764A double mutations did not differ in the other groups (P >0.05). Viral quasispecies diversity was lower in HIV/HBV co-infected patients than in HBV mono-infected patients (P <0.05). This study revealed the characteristic of HBV BCP/preC gene mutations and viral quasispecies diversity in HIV/HBV co-infected patients.

引用

页码：18 / 26

页数：9

共 25 条

[1] Sanger sequencing versus INNO-LiPA® HBV PreCore assay for routine detection of precore and basal core promoter mutations in hepatitis virus B chronically infected patients [J].