A continuous flow microfluidic-MS system for efficient OBOC screening

被引：5

作者：

Wang, Weizhi ^{[1
]}

Wei, Zewen ^{[1
]}

Wang, Zihua ^{[1
]}

Ma, Huailei ^{[1
]}

Bu, Xiangli ^{[1
]}

Hu, Zhiyuan ^{[1
,2
]}

机构：

[1] Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Beijing Inst Radiat Med, Beijing Proteome Res Ctr, Beijing 102206, Peoples R China

来源：

RSC ADVANCES | 2014年 / 4卷 / 106期

基金：

中国国家自然科学基金;

关键词：

COMBINATORIAL LIBRARIES; CHEMICAL LIBRARIES; BEAD; IDENTIFICATION; PEPTIDES; INTEGRATION; CHEMISTRY; DISCOVERY; LIGANDS; PROTEIN;

D O I：

10.1039/c4ra12911c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 10(5) beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.

引用

页码：61767 / 61770

页数：4

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