A continuous flow microfluidic-MS system for efficient OBOC screening

被引：5

作者：

Wang, Weizhi ^{[1
]}

Wei, Zewen ^{[1
]}

Wang, Zihua ^{[1
]}

Ma, Huailei ^{[1
]}

Bu, Xiangli ^{[1
]}

Hu, Zhiyuan ^{[1
,2
]}

机构：

[1] Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Beijing Inst Radiat Med, Beijing Proteome Res Ctr, Beijing 102206, Peoples R China

来源：

RSC ADVANCES | 2014年 / 4卷 / 106期

基金：

中国国家自然科学基金;

关键词：

COMBINATORIAL LIBRARIES; CHEMICAL LIBRARIES; BEAD; IDENTIFICATION; PEPTIDES; INTEGRATION; CHEMISTRY; DISCOVERY; LIGANDS; PROTEIN;

D O I：

10.1039/c4ra12911c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 10(5) beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.

引用

收藏

页码：61767 / 61770

页数：4

相关论文

共 35 条

[1] From combinatorial chemistry to cancer-targeting peptides [J].

Aina, Olulanu H. ;

Liu, Ruiwu ;

Sutcliffe, Julie L. ;

Marik, Jan ;

Pan, Chong-Xian ;

Lam, Kit S. .

MOLECULAR PHARMACEUTICS, 2007, 4 (05) :631-651

[2] Seamless Bead to Microarray Screening: Rapid Identification of the Highest Affinity Protein Ligands from Large Combinatorial Libraries [J].

Astle, John M. ;

Simpson, Levi S. ;

Huang, Yong ;

Reddy, M. Muralidhar ;

Wilson, Rosemary ;

Connell, Steven ;

Wilson, Johnnie ;

Kodadek, Thomas .

CHEMISTRY & BIOLOGY, 2010, 17 (01) :38-45

[3] Toward larger chemical libraries: Encoding with fluorescent colloids in combinatorial chemistry [J].

Battersby, BJ ;

Bryant, D ;

Meutermans, W ;

Matthews, D ;

Smythe, ML ;

Trau, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (09) :2138-2139

[4] CD13/APN is activated by angiogenic signals and is essential for capillary tube formation [J].

Bhagwat, SV ;

Lahdenranta, J ;

Giordano, R ;

Arap, W ;

Pasqualini, R ;

Shapiro, LH .

BLOOD, 2001, 97 (03) :652-659

[5] High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans [J].

Burns, Andrew R. ;

Kwok, Trevor C. Y. ;

Howard, Al ;

Houston, Ed ;

Johanson, Karl ;

Chan, Anthony ;

Cutler, Sean R. ;

McCourt, Peter ;

Roy, Peter J. .

NATURE PROTOCOLS, 2006, 1 (04) :1906-1914

[6] Multiple sequence alignment with the Clustal series of programs [J].

Chenna, R ;

Sugawara, H ;

Koike, T ;

Lopez, R ;

Gibson, TJ ;

Higgins, DG ;

Thompson, JD .

NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3497-3500

[7] Fabrication of nanopillars by nanosphere lithography [J].

Cheung, CL ;

Nikolic, RJ ;

Reinhardt, CE ;

Wang, TF .

NANOTECHNOLOGY, 2006, 17 (05) :1339-1343

[8] Discovery of Novel Integrin Ligands from Combinatorial Libraries Using a Multiplex "Beads on a Bead" Approach [J].

Cho, Choi-Fong ;

Amadei, Giulio A. ;

Breadner, Daniel ;

Luyt, Leonard G. ;

Lewis, John D. .

NANO LETTERS, 2012, 12 (11) :5957-5965

[9] Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex [J].

Choi, Youngseon ;

Lee, Junghan ;

Kim, Keumhyun ;

Kim, Heeyeon ;

Sommer, Peter ;

Song, Rita .

CHEMICAL COMMUNICATIONS, 2010, 46 (48) :9146-9148

[10] Utility of Redundant Combinatorial Libraries in Distinguishing High and Low Quality Screening Hits [J].

Doran, Todd M. ;

Gao, Yu ;

Mendes, Kimberly ;

Dean, Sonja ;

Simanski, Scott ;

Kodadek, Thomas .

ACS COMBINATORIAL SCIENCE, 2014, 16 (06) :259-270

← 1 2 3 4 →