Heterodimeric V. nikolskii phospholipases A2 induce aggregation of the lipid bilayer

被引：11

作者：

Alekseeva, A. S. ^{[1
]}

Tretiakova, D. S. ^{[1
]}

Chernikov, V. P. ^{[2
]}

Utkin, Yu. N. ^{[1
]}

Molotkovsky, J. G. ^{[1
]}

Vodovozova, E. L. ^{[1
]}

Boldyrev, I. A. ^{[1
,3
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Ul Miklukho Maklaya 16-10, Moscow 117997, Russia

[2] Res Inst Human Morphol, 3 Tsurupa St, Moscow 117418, Russia

[3] Pirogov Russian Natl Res Med Univ, 1 Ostrovityanov St, Moscow 117997, Russia

来源：

TOXICON | 2017年 / 133卷

基金：

俄罗斯基础研究基金会;

关键词：

Heterodimeric PLA2; FRET; Fluorescent lipid probes; Bilayer aggregation; NEUROTOXIC COMPLEX; VIPERA-NIKOLSKII; BETA-BUNGAROTOXIN; ANTIVENOM THERAPY; CRYSTAL-STRUCTURE; SNAKE-VENOM; A(2); CROTOXIN; TOXICITY; MECHANISM;

D O I：

10.1016/j.toxicon.2017.05.015

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We report that the action of the heterodimeric phospholipases A2 (PLA2s) from Vipera nikolskii, which comprises enzymatically active basic subunit and inactive acidic PLA2 homologue, on the lipid bilayer results in the aggregation and stacking of bilayers. These processes are demonstrated using two independent methods (fluorescence spectroscopy and electron microscopy). Aggregation of bilayers is possible because both subunits of the V. nikolskii heterodimer contain a membrane-binding site (also known as IBS). Thus, when the two IBSs bind to the membrane, the heterodimer acts as a connecting agent. Heterodimers induce aggregation of negatively charged bilayers composed of phosphatidylglycerol and do not induce aggregation of neutral bilayers composed of phosphatidylcholine. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：169 / 179

页数：11

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