Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India

被引：63

作者：

Akiba, Masato ^{[1
,2
]}

Sekizuka, Tsuyoshi ^{[3
]}

Yamashita, Akifumi ^{[3
]}

Kuroda, Makoto ^{[3
]}

Fujii, Yuki ^{[4
]}

Murata, Misato ^{[5
]}

Lee, Ken-ichi ^{[1
]}

Joshua, Derrick Ian ^{[6
]}

Balakrishna, Keshava ^{[6
]}

Bairy, Indira ^{[7
]}

Subramanian, Kaushik ^{[8
]}

Krishnan, Padma ^{[8
]}

Munuswamy, Natesan ^{[9
]}

Sinha, Ravindra K. ^{[10
]}

Iwata, Taketoshi ^{[1
]}

Kusumoto, Masahiro ^{[1
]}

Guruge, Keerthi S. ^{[11
]}

机构：

[1] Natl Agr & Food Res Org, Natl Inst Anim Hlth, Bacterial & Parasit Dis Res Div, Ibaraki, Japan

[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Osaka, Japan

[3] Natl Inst Infect Dis, Pathogen Genom Ctr, Tokyo, Japan

[4] Kenhoku Livestock Hyg Serv Ctr Ibaraki Prefecture, Ibaraki, Japan

[5] Chuo Livestock Hyg Serv Ctr Kumamoto Prefecture, Kumamoto, Japan

[6] Manipal Univ, Manipal Inst Technol, Dept Civil Engn, Manipal, India

[7] Manipal Univ, Melaka Manipal Med Coll, Dept Microbiol, Manipal, India

[8] Univ Madras, Dr ALM PG Inst Basic Med Sci, Dept Microbiol, Madras, Tamil Nadu, India

[9] Univ Madras, Dept Zool, Madras 600025, Tamil Nadu, India

[10] Univ Patna, Dept Zool, Patna 800005, Bihar, India

[11] Natl Agr & Food Res Org, Natl Inst Anim Hlth, Pathol & Pathophysiol Res Div, Ibaraki, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2016年 / 60卷 / 05期

基金：

日本学术振兴会;

关键词：

METALLO-BETA-LACTAMASE; HOSPITAL WASTE-WATER; QUINOLONE RESISTANCE; MOLECULAR CHARACTERIZATION; ENVIRONMENT; DISSEMINATION; GENES; IDENTIFICATION; SALMONELLA; BLA(NDM-1);

D O I：

10.1128/AAC.01950-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To determine the distribution and relationship of antimicrobial resistance determinants among extended-spectrum-cephalosporin (ESC)-resistant or carbapenem-resistant Escherichia coli isolates from the aquatic environment in India, water samples were collected from rivers or sewage treatment plants in five Indian states. A total of 446 E. coli isolates were randomly obtained. Resistance to ESC and/or carbapenem was observed in 169 (37.9%) E. coli isolates, which were further analyzed. These isolates showed resistance to numerous antimicrobials; more than half of the isolates exhibited resistance to eight or more antimicrobials. The bla(NDM) gene was detected in 14/21 carbapenem-resistant E. coli isolates: bla(NDM-1) in 2 isolates, bla(NDM-5) in 7 isolates, and bla(NDM-7) in 5 isolates. The bla(CTX-M) gene was detected in 112 isolates (66.3%): bla(CTX-M-15) in 108 isolates and bla(CTX-M-55) in 4 isolates. We extracted 49 plasmids from selected isolates, and their whole-genome sequences were determined. Fifty resistance genes were detected, and 11 different combinations of replicon types were observed among the 49 plasmids. The network analysis results suggested that the plasmids sharing replicon types tended to form a community, which is based on the predicted gene similarity among the plasmids. Four communities each containing from 4 to 17 plasmids were observed. Three of the four communities contained plasmids detected in different Indian states, suggesting that the interstate dissemination of ancestor plasmids has already occurred. Comparison of the DNA sequences of the bla(NDM)-positive plasmids detected in this study with known sequences of related plasmids suggested that various mutation events facilitated the evolution of the plasmids and that plasmids with similar genetic backgrounds have widely disseminated in India.

引用

页码：2972 / 2980

页数：9

共 43 条

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