Quantitative endogenous formate analysis in plasma using headspace gas chromatography without a headspace analyzer

被引:13
作者
Sokoro, AbdulRazaq
Lehotay, Denis
Eichhorst, Jeff
Treble, Ronald
机构
[1] Saskatchewan Dis Control Lab, Regina, SK S4S 5W6, Canada
[2] Univ Regina, Regina, SK S4S 0A2, Canada
关键词
D O I
10.1093/jat/31.6.342
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The objective was to develop a simple routine method for quantitative measurement of endogenous formic acid in plasma and whole blood using headspace gas chromatography-flame ionization detection. (GC-FID). Two-hundred microliters of sample was placed in a 1-mL glass vial. Fifty microliters of aqueous ethanol (10%) was added as an internal standard and a derivatizing agent. Ethylformate formation was enhanced by addition of 200 μL concentrated sulfuric acid as a catalyst. The vials were then sealed immediately and placed in a water bath for 15 min at 60°C. One milliliter of this headspace gas was siphoned using a gas-tight syringe and injected into a GC-FID fitted with a capillary column. Ethanol eluted at approximately 3.0 min, and ethylformate eluted around 4.7 min. The limit of quantitation for ethylformate was 0.026 mmol/L, and the limit of detection was 0.020 mmol/L. Imprecisions for spiked plasma samples at 0.25 and 1 mmol/L were 10% and 9%, respectively, and recoveries were at 100% and 108%, respectively. A simple, reliable, and highly specific headspace analysis method for quantifying endogenous formate without the use of a headspace analyzer was developed. This method enables the routine clinical analysis of formate in plasma and whole blood samples.
引用
收藏
页码:342 / 346
页数:5
相关论文
共 21 条
[1]   OSMOLAL AND ANION GAPS IN PATIENTS ADMITTED TO AN EMERGENCY MEDICAL DEPARTMENT [J].
AABAKKEN, L ;
JOHANSEN, KS ;
RYDNINGEN, EB ;
BREDESEN, JE ;
OVREBO, S ;
JACOBSEN, D .
HUMAN & EXPERIMENTAL TOXICOLOGY, 1994, 13 (02) :131-134
[2]   GAS-CHROMATOGRAPHIC HEADSPACE ASSAY OF FORMIC-ACID AS METHYL FORMATE IN BIOLOGIC FLUIDS - POTENTIAL APPLICATION TO METHANOL POISONING [J].
ABOLIN, C ;
MCRAE, JD ;
TOZER, TN ;
TAKKI, S .
BIOCHEMICAL MEDICINE, 1980, 23 (02) :209-218
[3]  
Barceloux DG, 2002, J TOXICOL-CLIN TOXIC, V40, P415
[4]   A SIMPLE ENZYMATIC METHOD FOR THE MEASUREMENT OF ABNORMAL LEVELS OF FORMATE IN PLASMA [J].
BUTTERY, JE ;
CHAMBERLAIN, BR .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1988, 12 (05) :292-294
[5]   Methanol toxicity and formate oxidation in NEUT2 mice [J].
Cook, RJ ;
Champion, KM ;
Giometti, CS .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 393 (02) :192-198
[6]   PHARMACOKINETICS OF INHALED [C-14] METHANOL AND METHANOL-DERIVED [C-14] FORMATE IN NORMAL AND FOLATE-DEFICIENT CYNOMOLGUS MONKEYS [J].
DORMAN, DC ;
MOSS, OR ;
FARRIS, GM ;
JANSZEN, D ;
BOND, JA ;
MEDINSKY, MA .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1994, 128 (02) :229-238
[7]  
ELLENHORN MJ, 1997, ELLENHORNS MED TOXIC, P1127
[8]   The role of serine hydroxymethyltransferase isozymes in one-carbon metabolism in MCF-7 cells as determined by 13C NMR [J].
Fu, TF ;
Rife, JP ;
Schirch, V .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 393 (01) :42-50
[9]   Absorption and elimination of formate following oral administration of calcium formate in female human subjects [J].
Hanzlik, RP ;
Fowler, SC ;
Eells, JT .
DRUG METABOLISM AND DISPOSITION, 2005, 33 (02) :282-286
[10]   Increased serum formate in the diagnosis of methanol poisoning [J].
Hovda, KE ;
Urdal, P ;
Jacobsen, D .
JOURNAL OF ANALYTICAL TOXICOLOGY, 2005, 29 (06) :586-588