Inhibition of the protease activity of the light chain of type A botulinum neurotoxin by aqueous extract from stinging nettle (Urtica dioica) leaf

被引：10

作者：

Gul, N ^{[1
]}

Ahmed, SA ^{[1
]}

Smith, LA ^{[1
]}

机构：

[1] USA, Med Res Inst Infect Dis, Dept Immunol & Mol Biol, Div Toxinol & Aerobiol, Ft Detrick, MD 21702 USA

来源：

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY | 2004年 / 95卷 / 05期

关键词：

D O I：

10.1111/j.1742-7843.2004.pto950503.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We investigated the inhibitory effect of stinging nettle leaf extract on the protease activity of botulinum neurotoxin type A and B light chains. The nettle leaf infusion was fractionated and HPLC-based enzymatic assays were performed to determine the capacity of each fraction to inhibit the protease activity of botulinum neurotoxin type A and B light chains. Assay results demonstrated that a water-soluble fraction obtained from the nettle leaf infusion inhibited type A, but did not inhibit type B light chain protease activity. The inhibition mode of water soluble fraction against protease activity of type A light chain was analyzed and found to be a non-competitive.

引用

页码：215 / 219

页数：5