Inhibition of the protease activity of the light chain of type A botulinum neurotoxin by aqueous extract from stinging nettle (Urtica dioica) leaf

被引：10
|
作者：
Gul, N [1 ]
Ahmed, SA [1 ]
Smith, LA [1 ]
机构：
[1] USA, Med Res Inst Infect Dis, Dept Immunol & Mol Biol, Div Toxinol & Aerobiol, Ft Detrick, MD 21702 USA
来源：
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY | 2004年 / 95卷 / 05期
关键词：
D O I：
10.1111/j.1742-7843.2004.pto950503.x
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
We investigated the inhibitory effect of stinging nettle leaf extract on the protease activity of botulinum neurotoxin type A and B light chains. The nettle leaf infusion was fractionated and HPLC-based enzymatic assays were performed to determine the capacity of each fraction to inhibit the protease activity of botulinum neurotoxin type A and B light chains. Assay results demonstrated that a water-soluble fraction obtained from the nettle leaf infusion inhibited type A, but did not inhibit type B light chain protease activity. The inhibition mode of water soluble fraction against protease activity of type A light chain was analyzed and found to be a non-competitive.
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收藏
页码：215 / 219
页数：5
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